Single-molecule mRNA decay measurements reveal promoter- regulated mRNA stability in yeast.
Single-molecule mRNA decay measurements reveal promoter- regulated mRNA stability in yeast.
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DOI：
10.1016/j.cell.2011.11.051
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发表时间：
2011-12-23
期刊：
影响因子：
64.5
通讯作者：
Singer RH
中科院分区：
文献类型：
--
作者：
Trcek T;Larson DR;Moldón A;Query CC;Singer RH
信使 RNA 衰减测量通常在细胞群上进行。然而，这种方法无法揭示足够的复杂性，无法提供有关可能在短时间内调节 mRNA 降解的机制的信息。为了解决这一缺陷，我们使用单分子 FISH 测量了单个酵母细胞中细胞周期调节的衰变。我们发现负责有丝分裂进展的两个基因 SWI5 和 CLB2 表现出有丝分裂依赖性 mRNA 稳定性开关。它们的转录本在有丝分裂之前一直保持稳定，此时急剧的衰变消除了 mRNA 补体，从而防止进入下一个周期。值得注意的是，衰变的特异性和时间完全由它们的启动子调节，与特定的顺式 mRNA 序列无关。有丝分裂出口网络蛋白 Dbf2p 与 SWI5 和 CLB2 mRNA 共转录结合并调节它们的衰变。这项工作揭示了 mRNA 稳定性的启动子依赖性控制，这是一种可被多种 mRNA 和生物体采用的新型调节机制。
Messenger RNA decay measurements are typically performed on a population of cells. However, this approach cannot reveal sufficient complexity to provide information on mechanisms that may regulate mRNA degradation, possibly on short time scales. To address this deficiency, we measured cell cycle regulated decay in single yeast cells using single-molecule FISH. We found that two genes responsible for mitotic progression, SWI5 and CLB2 exhibit a mitosis-dependent mRNA stability switch. Their transcripts are stable until mitosis when a precipitous decay eliminates the mRNA complement, preventing carry-over into the next cycle. Remarkably, the specificity and timing of decay is entirely regulated by their promoter, independent of specific cis mRNA sequences. The mitotic exit network protein, Dbf2p binds to SWI5 and CLB2 mRNAs co-transcriptionally and regulates their decay. This work reveals the promoter-dependent control of mRNA stability, a novel regulatory mechanism that could be employed by a variety of mRNAs and organisms.
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DOI：
10.1126/science.1202142
发表时间：
2011-04-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Larson DR;Zenklusen D;Wu B;Chao JA;Singer RH
通讯作者：
Singer RH
影响因子：
5.3
作者：
Clement, Sandra L.;Scheckel, Claudia;Lykke-Andersen, Jens
通讯作者：
Lykke-Andersen, Jens
影响因子：
11.4
作者：
Liu, HY;Toyn, JH;Denis, CL
通讯作者：
Denis, CL
影响因子：
56.9
作者：
Femino, A;Fay, FS;Singer, RH
通讯作者：
Singer, RH
影响因子：
4.5
作者：
Niu W;Li Z;Zhan W;Iyer VR;Marcotte EM
通讯作者：
Marcotte EM