Selection and application of aptamers with high-affinity and high-specificity against dinophysistoxin-1.
Selection and application of aptamers with high-affinity and high-specificity against dinophysistoxin-1.
复制标题
Dinophystoxin-1高亲和力、高特异性核酸适配体的筛选及应用
作者：
腹泻性贝类毒素(DSTs)是广泛分布于世界各地的海洋毒素，对人类健康构成重大威胁。Dinophysistoxin-1(DTX-1)在DST中具有最强的毒性。然而，目前的检测方法存在伦理问题和技术缺陷。还需要进一步的研究，以开发出更适合于监管制度的替代方案。在这项工作中，我们首次成功地获得了与DTX-1结合的高亲和力和高特异性的适体。经过优化，获得了NM的高kD适体的核心序列，并探索了该核心序列与Dtx-1的结合方式。基于该适配子，我们开发了一种用于DTX-1检测的生物层干涉计量(BLI)生物传感器平台。该传感器的检测范围为40~600 nM DTX-1(线性范围为80~200 nM)，最低检测下限为614 pM此外，该适配子传感器具有良好的重复性和稳定性，表明该适配子传感器在DTX-1的灵敏快速检测中具有广阔的发展前景。
Diarrhetic shellfish toxins (DSTs) are marine toxins distributed widely in the world, which pose a major threat to the health of mankind. Dinophysistoxin-1 (DTX-1) has the most potent toxicity in DSTs. However, the current detection methods have ethical problems and technical defects. Further research is needed, to develop a more suitable alternative to the supervision system. In this work, we successfully obtained an aptamer with high affinity and specificity bound to DTX-1 for the first time. After optimization, a core sequence of the aptamer with a higher KD of 64 nM was obtained, while the binding mode of the core sequence and DTX-1 was explored. Based on this aptamer, we developed a biolayer interferometry (BLI) biosensor platform for DTX-1 detection. The aptasensor exhibited a broad detection range from 40 to 600 nM DTX-1 (linear range from 80 to 200 nM), and the low detection limit was 614 pM. Morever, the aptasensor showed good reproducibility and stability, which indicated that this novel aptasensor had broad development prospects for the sensitive and rapid detection of DTX-1.
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影响因子：
4.5
作者：
Akter S;Vehniäinen M;Kankaanpää HT;Lamminmäki U
通讯作者：
Lamminmäki U
影响因子：
3.3
作者：
通讯作者：
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影响因子：
12.6
作者：
Gao, Ya;Zhu, Ziyu;Wang, Shengfu
通讯作者：
Wang, Shengfu
DOI：
10.2174/138620709789104915
发表时间：
2009-09-01
影响因子：
1.8
作者：
Concepcion, Joy;Witte, Krista;Tan, Hong
通讯作者：
Tan, Hong
影响因子：
12.6
作者：
Gao, Shunxiang;Hu, Bo;Wang, Lianghua
通讯作者：
Wang, Lianghua