Spirillospora tritici sp. nov., a Novel Actinomycete Isolated from Rhizosphere Soil of Triticum aestivum L.
Spirillospora tritici sp. nov., a Novel Actinomycete Isolated from Rhizosphere Soil of Triticum aestivum L.
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小麦螺旋孢子菌
DOI：
10.1007/s00284-018-1548-3
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发表时间：
2018-08
影响因子：
2.6
通讯作者：
Wensheng Xiang
中科院分区：
文献类型：
--
作者：
Jing Song;Jiabin Wang;Tianyu Sun;Chuang Li;Hairong He;Linlin Shi;Xiaowei Guo;Junwei Zhao;Wensheng Xiang
从小麦根际土壤中分离到一株革兰氏阳性好氧放线菌，命名为SJ 21 T。采自中国中部河北省廊坊。抗菌活性较弱的菌株SJ 21 T还含有非核糖体肽合成酶（NRPS）、酮合酶（KS）和甲基丙二酰转移酶结构域（PKS-I）以及KSα和KSβ结构域（PKS-II）基因。对SJ 21 T菌株进行了多相分类学研究。该菌株在气生菌丝上形成球形孢子囊泡（7.0-8.9 µm），由卷曲和分支链组成。16 SrRNA基因序列分析表明，SJ 21 T属于螺孢菌属，与其最近邻的白色螺孢菌IFO 12248 T形成明显的分支（98.7%）。该菌株的形态学和化学分类学特征也与螺孢菌属的成员一致。对SJ 21 T和S. albida，进一步阐明了它们的亲缘关系，并证明SJ 21 T与S. albida。因此，该菌株被认为代表了螺孢菌属的一个新种，并建议将其命名为三孢螺孢菌新种。模式菌株为SJ 21 T（=CGMCC 4.7420 T =JCM 32390 T）。
A Gram-positive, aerobic actinomycetes, designated strain SJ 21T, was isolated from the rhizosphere soil of Triticum aestivum L. collected from Langfang, Hebei Province, Central China. Strain SJ 21T with weak antifungal activity also contained genes (involved in antibiotics biosynthesis) of the nonribosomal peptide synthetases (NRPS), ketosynthase (KS) and methyl malonyl transferase domains (PKS-I) as well as KSα and KSβ domains (PKS-II). A polyphasic taxonomic study was carried out to establish the status of strain SJ 21T. The strain formed spherical spore vesicles (7.0–8.9 µm) consisting of coiled and branched chains on aerial mycelia. 16S rRNA gene sequence similarity studies showed that strain SJ 21T belongs to the genus Spirillospora and formed a distinct branch with its closest neighbour Spirillospora albida IFO 12248T (98.7%). The morphological and chemotaxonomic properties of the strain are also consistent with those members of the genus Spirillospora. DNA–DNA hybridization experiments and phenotypic tests were carried out between strain SJ 21T and S. albida, which further clarified their relatedness and demonstrated that SJ 21T could be distinguished genomically from S. albida. Therefore, the strain is considered to represent a novel species of the genus Spirillospora, for which the name Spirillospora tritici sp. nov. is proposed. The type strain is SJ 21T (=CGMCC 4.7420T =JCM 32390T).
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DOI：
10.1002/jobm.19700100205
发表时间：
1970
期刊：
Zeitschrift fur allgemeine Mikrobiologie
影响因子：
--
作者：
T. McInnis;A. Domnas
通讯作者：
T. McInnis;A. Domnas
DOI：
10.1099/00207713-51-2-373
发表时间：
2001-03
影响因子：
2.8
作者：
Z. Zhang;T. Kudo;Y. Nakajima;Y. Wang
通讯作者：
Z. Zhang;T. Kudo;Y. Nakajima;Y. Wang
DOI：
10.1111/j.1432-1033.1970.tb00830.x
发表时间：
1970
期刊：
European journal of biochemistry
影响因子：
--
作者：
J. Ley;H. Cattoir;A. Reynaerts
通讯作者：
J. Ley;H. Cattoir;A. Reynaerts
DOI：
--
发表时间：
1961
期刊：
--
影响因子：
--
作者：
S. Waksman
通讯作者：
S. Waksman
DOI：
10.1016/0385-6380(87)90108-7
发表时间：
1987-10-01
期刊：
JOURNAL OF FERMENTATION TECHNOLOGY
影响因子：
--
作者：
HAYAKAWA, M;NONOMURA, H
通讯作者：
NONOMURA, H