Serological studies with influenza A(H1N1) viruses cultivated in eggs or in a canine kidney cell line (MDCK).
Serological studies with influenza A(H1N1) viruses cultivated in eggs or in a canine kidney cell line (MDCK).
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对鸡蛋或犬肾细胞系 (MDCK) 中培养的甲型 H1N1 流感病毒进行血清学研究。
DOI：
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发表时间：
1987
影响因子：
11.1
通讯作者：
G. Schild
中科院分区：
文献类型：
--
作者：
J. Oxford;T. Corcoran;R. Knott;J. Bates;D. Major;R. Newman;P. Yates;J. Robertson;R. Webster;G. Schild
从犬肾（MDCK）细胞或母鸡胚蛋中培养的同一临床标本中培养的甲型H1N1流感病毒对，通常可以通过它们与血凝素单克隆抗体以及与雪貂或人血清中的抗体的反应来区分。在MDCK培养物中进一步传代的鸡蛋适应病毒在血清学特征上仍保持“鸡蛋样”，这表明它们的血清学反应的差异不是宿主细胞依赖性血凝素糖基化的直接结果。用MDCK细胞中单独生长的病毒进行的试验，比用适应胚胎卵生长的病毒进行的试验，可以更频繁地检测到人血清中的血凝抑制（HI）或病毒中和抗体，并且检测到更高的滴度。在1983年分离出的一系列适应在鸡蛋中生长的甲型H1N1流感病毒毒株对感染了卵生长病毒的雪貂的血清进行检测时，在HI试验的血清学反应中发现了显著的差异。相比之下，感染MDCK衍生病毒的雪貂血清在血清学上无法区分仅在MDCK细胞中传代的相同病毒，并且在鸡蛋适应的对应病毒之间也显示出相对较小的差异。结论是，在解释甲型H1N1流感分离株的菌株特征结果和使用这些病毒进行血清调查时，用于病毒分离和培养的细胞底物是一个应考虑的因素。
Pairs of influenza A(H1N1) viruses cultivated from the same clinical specimen in canine kidney (MDCK) cells or in embryonated hens' eggs can frequently be distinguished by their reactions with monoclonal antibodies to haemagglutinin and with antibodies in ferret or human sera. Egg-adapted virus, further passaged in MDCK cultures remained "egg-like" in serological characteristics indicating that the differences in their serological reactions were not a direct result of host cell-dependent glycosylation of the haemagglutinin. Haemagglutination-inhibiting (HI) or virus neutralizing antibodies in human sera can be detected more frequently, and to higher titre, in tests employing virus grown exclusively in MDCK cells than in tests with virus adapted to growth in embryonated eggs. Striking differences were detected in the serological reactions in HI tests when sera from ferrets infected with egg-grown virus were tested against a series of strains of influenza A(H1N1) virus isolated in 1983 and adapted to growth in eggs. In contrast, sera from ferrets infected with MDCK-derived virus failed to distinguish serologically between the same viruses that had been passaged exclusively in MDCK cells and also revealed relatively small differences between their egg-adapted counterparts.It was concluded that the cell substrate used for virus isolation and cultivation is a factor that should be considered when interpreting the results of strain characterization of influenza A(H1N1) isolates and in sero-surveys using these viruses.
影响因子：
56.9
作者：
HOLLAND, J;SPINDLER, K;VANDEPOL, S
通讯作者：
VANDEPOL, S
流感病毒在含胚卵或组织培养物中的连续传代：突变体的出现。
影响因子：
3.7
作者：
Brand,C;Palese,P
通讯作者：
Palese,P
乙型流感病毒的异质性。
影响因子：
11.1
作者：
Bao-Lan,L;Webster,RG;Brown,LE;Nerome,K
通讯作者：
Nerome,K