The clock gene circuit in Arabidopsis includes a repressilator with additional feedback loops.
The clock gene circuit in Arabidopsis includes a repressilator with additional feedback loops.
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DOI：
10.1038/msb.2012.6
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发表时间：
2012-03-06
影响因子：
9.9
通讯作者：
Millar, Andrew J.
中科院分区：
文献类型：
--
作者：
Pokhilko, Alexandra;Fernandez, Aurora Pinas;Edwards, Kieron D.;Southern, Megan M.;Halliday, Karen J.;Millar, Andrew J.
生物钟利用包括互锁的转录反馈环在内的分子机制，使生物过程与昼夜周期同步。最近的实验发现，晚上复合物（EC）作为一个阻遏物，可以是必不可少的基因表达节奏在植物中。整合EC组件在这个角色中显着改变了我们的机械，时钟基因电路的数学模型。EC基因的负向自动调节构成了时钟的晚循环，取代了假设的Y成分。EC解释了我们先前的推测，即早晨基因假反应调节因子9被一个晚上基因抑制，该基因先前被鉴定为CAB表达的时间1（TOC1）。我们的计算分析表明，TOC1是早晨基因LATE ELLONGATED HYPOCOTYL和CIRCADIAN CLOCK ASSOCIATED 1的阻遏物，而不是最初设想的激活物。这消除了先前时钟模型中未知分量X（或TOC1mod）的必要性。以及匹配的时间序列和相位响应数据，该模型提供了一个新的概念框架的工厂时钟，包括一个三组件的再加压电路在其复杂的结构。
Circadian clocks synchronise biological processes with the day/night cycle, using molecular mechanisms that include interlocked, transcriptional feedback loops. Recent experiments identified the evening complex (EC) as a repressor that can be essential for gene expression rhythms in plants. Integrating the EC components in this role significantly alters our mechanistic, mathematical model of the clock gene circuit. Negative autoregulation of the EC genes constitutes the clock's evening loop, replacing the hypothetical component Y. The EC explains our earlier conjecture that the morning gene PSEUDO-RESPONSE REGULATOR 9 was repressed by an evening gene, previously identified with TIMING OF CAB EXPRESSION1 (TOC1). Our computational analysis suggests that TOC1 is a repressor of the morning genes LATE ELONGATED HYPOCOTYL and CIRCADIAN CLOCK ASSOCIATED1 rather than an activator as first conceived. This removes the necessity for the unknown component X (or TOC1mod) from previous clock models. As well as matching timeseries and phase-response data, the model provides a new conceptual framework for the plant clock that includes a three-component repressilator circuit in its complex structure.
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影响因子：
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