UGT74D1 catalyzes the glucosylation of 2-oxindole-3-acetic acid in the auxin metabolic pathway in Arabidopsis.
UGT74D1 catalyzes the glucosylation of 2-oxindole-3-acetic acid in the auxin metabolic pathway in Arabidopsis.
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DOI：
10.1093/pcp/pct173
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发表时间：
2014-01
影响因子：
4.9
通讯作者：
Kasahara H
中科院分区：
文献类型：
--
作者：
Tanaka K;Hayashi K;Natsume M;Kamiya Y;Sakakibara H;Kawaide H;Kasahara H
IAA是一种天然存在的生长素，在调节植物生长和发育中起着至关重要的作用。植物内源IAA的浓度受其生物合成、运输和失活的时空调控。已有研究表明，IAA代谢为2-羟吲哚-3-乙酸（OxIAA）和OxIAA-Glc（OxIAA-Glc）可能在IAA体内平衡中发挥重要作用，但参与这一代谢途径的基因尚不清楚。在这项研究中，我们表明，UGT 74 D1催化拟南芥中OxIAA的糖基化。通过筛选转化了拟南芥UDP-糖基转移酶（UGT）基因的酵母菌，我们发现在表达UGT 74 D1的酵母菌培养基中，存在OxIAA时，OxIAA-Glc在培养基中积累。此外，我们表明在大肠杆菌中表达的UGT 74 D1将OxIAA转化为OxIAA-Glc。UGT 74 D1缺陷突变体中OxIAA-Glc的内源浓度降低了85%，而OxIAA的内源浓度增加了2.5倍，表明UGT 74 D1在OxIAA代谢中起主要作用。此外，UGT 74 D1的诱导显著增加了OxIAA-Glc的水平和根向地性的丧失。这些结果表明UGT 74 D1催化拟南芥中OxIAA依赖性IAA代谢途径中的一个关键步骤。
IAA is a naturally occurring auxin that plays a crucial role in the regulation of plant growth and development. The endogenous concentration of IAA is spatiotemporally regulated by biosynthesis, transport and its inactivation in plants. Previous studies have shown that the metabolism of IAA to 2-oxindole-3-acetic acid (OxIAA) and OxIAA-glucoside (OxIAA-Glc) may play an important role in IAA homeostasis, but the genes involved in this metabolic pathway are still unknown. In this study, we show that UGT74D1 catalyzes the glucosylation of OxIAA in Arabidopsis. By screening yeasts transformed with Arabidopsis UDP-glycosyltransferase (UGT) genes, we found that OxIAA-Glc accumulates in the culture media of yeasts expressing UGT74D1 in the presence of OxIAA. Further, we showed that UGT74D1 expressed in Escherichia coli converts OxIAA to OxIAA-Glc. The endogenous concentration of OxIAA-Glc decreased by 85% while that of OxIAA increased 2.5-fold in ugt74d1-deficient mutants, indicating the major role of UGT74D1 in OxIAA metabolism. Moreover, the induction of UGT74D1 markedly increased the level of OxIAA-Glc and loss of root gravitropism. These results indicate that UGT74D1 catalyzes a committed step in the OxIAA-dependent IAA metabolic pathway in Arabidopsis.
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DOI：
10.1073/pnas.260502697
发表时间：
2000-12-19
影响因子：
11.1
作者：
Barlier, I;Kowalczyk, M;Bellini, C
通讯作者：
Bellini, C
影响因子：
4.8
作者：
Li, Y;Baldauf, S;Bowles, DJ
通讯作者：
Bowles, DJ
影响因子：
7.2
作者：
Nakazawa, M;Yabe, N;Matsui, M
通讯作者：
Matsui, M
影响因子：
3.7
作者：
Jin SH;Ma XM;Han P;Wang B;Sun YG;Zhang GZ;Li YJ;Hou BK
通讯作者：
Hou BK
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF