Antibacterial activity and mechanism of ThDP analogs against rice brown stripe pathogen Acidovorax avenae subsp. avenae RS-1
Antibacterial activity and mechanism of ThDP analogs against rice brown stripe pathogen Acidovorax avenae subsp. avenae RS-1
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ThDP类似物对水稻褐纹病病菌燕麦酸食菌亚种的抗菌活性及作用机制
DOI：
10.1007/s42161-018-0137-4
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发表时间：
2018-08
影响因子：
2.2
通讯作者：
Sun Guo Chang
中科院分区：
文献类型：
--
作者：
Wang Xiao Xuan;Qi Hang Ying;Chen Jie;Yang Ying Zi;Qiu Wen;Wang Wei;Zou Peng;Li Bin;Wang Yan Li;He Hong Wu;Sun Guo Chang
根据大肠杆菌丙酮酸脱氢酶多酶复合体E1的ThDP结合部位，设计并合成了ThDP类似物。研究了66个新的ThDP类似物对水稻细菌性褐斑病病原菌Acidovorax venae亚种的影响。燕麦菌株RS-1。结果表明，66个ThDP类似物中的3个(分别命名为20、21和53)对RS-1菌株的体外生长有明显的抑制作用。但未发现该水稻病原菌有明显的细胞裂解和破坏现象，这与透射电子显微镜的形态证据一致。相反，这三个ThDP类似物显著减少了RS-1菌株的生物被膜形成和丙酮酸脱氢酶的活性。此外，利用实时定量聚合酶链式反应分析了ThDP靶基因和20个分泌系统相关基因的差异表达。其中，ThDP 20处理组的ThDP靶基因和VgrG-5的表达明显高于对照组，提示ThDP类似物的抗菌机制可能主要是通过改变ThDP靶基因和分泌系统相关基因的表达，而不是对细胞膜造成损伤。综上所述，应用合成的ThDP类似物可能是克服水稻细菌性褐斑病病原菌的一种有效策略。
Thiamin diphosphate (ThDP) analogs have been designed and synthesized based on the ThDP binding site of the pyruvate dehydrogenase multienzyme complex E1 of Escherichia coli. This study investigated the effect of 66 novel ThDP analogs on rice bacterial brown stripe pathogen Acidovorax avenae subsp. avenae strain RS-1. Results indicated that three of the 66 ThDP analogs (designated as 20, 21 and 53) significantly inhibited the in vitro growth of strain RS-1. However, no obvious cell lysis and destruction was found for this rice pathogenic bacterium, which were supported by morphological evidence of transmission electron microscope. In contrast, the three ThDP analogs significantly reduced biofilm formation and the activity of the pyruvate dehydrogenase of strain RS-1. Furthermore, the differential expression of ThDP target gene and 20 secretion system related genes were revealed by using quantitative real-time PCR. Among these, the expressions of ThDP target gene and VgrG-5 under the treatment of ThDP 20 were strongly induced compared to the control, which indicated that the antibacterial mechanism of ThDP analogs may be mainly due to the changed expression of ThDP target gene and secretion system related genes rather than causing damage to cell membrane. Taken together, the application of synthesized ThDP analogs might be a tractable strategy to overcome the pathogen of rice bacterial brown stripe.
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影响因子：
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DOI：
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发表时间：
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期刊：
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影响因子：
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