Evaluation of cholinesterase inhibitory and antioxidant activity of Wedelia chinensis and isolation of apigenin as an active compound.
Evaluation of cholinesterase inhibitory and antioxidant activity of Wedelia chinensis and isolation of apigenin as an active compound.
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DOI：
10.1186/s12906-021-03373-4
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发表时间：
2021-07-27
影响因子：
3.9
通讯作者：
Sadik G
中科院分区：
文献类型：
--
作者：
Islam MA;Zaman S;Biswas K;Al-Amin MY;Hasan MK;Alam AHMK;Tanaka T;Sadik G
据报道，中国野菜是一种民间药物，用于治疗包括神经退行性疾病在内的不同疾病。虽然这种植物的多种生物活性已经被研究得很好，但这种植物在神经系统疾病中的作用在很大程度上是未知的。摘要本研究旨在探讨白杨对胆碱酯酶的抑制作用和抗氧化能力。采用改进的Ellman法测定了该植物提取物和提取物的乙酰胆碱酯酶和丁基胆碱酯酶抑制活性。通过还原能力、总抗氧化能力、总酚类和类黄酮含量、清除2,2 ' -二苯基-1-吡啶肼（DPPH）自由基和羟基自由基、抑制脑脂质过氧化等体外模型/实验来评估其抗氧化活性。采用色谱法和波谱法对提取物中的活性成分进行分离鉴定。其中，水馏分（AQF）和乙酸乙酯馏分（EAF）对乙酰胆碱酯酶（IC50分别为40.02±0.16 μg/ml和57.76±0.37 μg/ml）和丁基胆碱酯酶（IC50分别为31.79±0.18 μg/ml和48.41±0.05 μg/ml）具有较强的抑制作用。同样，EAF和AQF的酚类物质和黄酮类物质含量较高，在DPPH和羟基自由基清除、还原力和总抗氧化活性等多项抗氧化实验中均具有较强的抗氧化活性。有效抑制脑脂过氧化作用，IC50值分别为45.20±0.10 μg/ml和25.53±0.04 μg/ml。总黄酮与抗氧化活性和抑制胆碱酯酶活性呈显著相关。活性引导色谱分离分离得到了一种主要的活性化合物，并通过光谱分析确定其结构为芹菜素。该植物抑制胆碱酯酶活性和脂质过氧化的潜在能力表明，该植物可能对阿尔茨海默病的管理有用。在线版本包含补充材料，可在10.1186/s12906-021- 03374 -4获得。
Wedelia chinensis has been reported as a folk medicine for the treatment of different diseases including neurodegenerative disease. Although the plant has been studied well for diverse biological activities, the effect of this plant in neurological disorder is largely unknown. The present study was undertaken to evaluate the cholinesterase inhibitory and antioxidant potential of W. chinensis. The extract and fractions of the plant were evaluated for acetylcholinesterase and butyrylcholinesterase inhibitory activity by modified Ellman method. The antioxidant activity was assessed in several in vitro models/assays such as reducing power, total antioxidant capacity, total phenolic and flavonoid content, scavenging of 2,2′-diphenyl-1-picrylhydrazyl (DPPH) free radical and hydroxyl radical, and inhibition of brain lipid peroxidation. Chromatographic and spectroscopic methods were used to isolate and identify the active compound from the extract. Among the fractions, aqueous fraction (AQF) and ethylacetate fraction (EAF) exhibited high inhibition against acetylcholinesterase (IC50: 40.02 ± 0.16 μg/ml and 57.76 ± 0.37 μg/ml) and butyrylcholinesterase (IC50: 31.79 ± 0.18 μg/ml and 48.41 ± 0.05 μg/ml). Similarly, the EAF and AQF had high content of phenolics and flavonoids and possess strong antioxidant activity in several antioxidant assays including DPPH and hydroxyl radical scavenging, reducing power and total antioxidant activity. They effectively inhibited the peroxidation of brain lipid in vitro with IC50 values of 45.20 ± 0.10 μg/ml and 25.53 ± 0.04 μg/ml, respectively. A significant correlation was observed between total flavonoids and antioxidant and cholinesterase inhibitory activity. Activity guided chromatographic separation led to the isolation of a major active compound from the EAF and its structure was elucidated as apigenin by spectral analysis. The potential ability of W. chinensis to inhibit the cholinesterase activity and peroxidation of lipids suggest that the plant might be useful for the management of AD. The online version contains supplementary material available at 10.1186/s12906-021-03373-4.
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影响因子：
48
作者：
Kalaria, Raj N.;Maestre, Gladys E.;Arizaga, Raul;Friedland, Robert P.;Galasko, Doug;Hall, Kathleen;Luchsinger, Jose A.;Ogunniyi, Adesola;Perry, Elaine K.;Potocnik, Felix;Prince, Martin;Stewart, Robert;Wimo, Anders;Zhang, Zhen-Xin;Antuono, Piero
通讯作者：
Antuono, Piero
影响因子：
5.8
作者：
ELLMAN, GL;COURTNEY, KD;FEATHERSTONE, RM
通讯作者：
FEATHERSTONE, RM
影响因子：
5.6
作者：
Ayaz M;Junaid M;Ullah F;Subhan F;Sadiq A;Ali G;Ovais M;Shahid M;Ahmad A;Wadood A;El-Shazly M;Ahmad N;Ahmad S
通讯作者：
Ahmad S
影响因子：
--
作者：
Ayaz M;Junaid M;Ahmed J;Ullah F;Sadiq A;Ahmad S;Imran M
通讯作者：
Imran M
影响因子：
4.7
作者：
Lin, Feng-Min;Chen, Li-Ru;Hsiao, Pei-Wen
通讯作者：
Hsiao, Pei-Wen