Investigation of human exposure to triclocarban after showering and preliminary evaluation of its biological effects.
Investigation of human exposure to triclocarban after showering and preliminary evaluation of its biological effects.
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DOI：
10.1021/es103650m
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发表时间：
2011-04-01
影响因子：
11.4
通讯作者：
Hammock, Bruce D.
中科院分区：
文献类型：
--
作者：
Schebb, Nils Helge;Inceoglu, Bora;Ahn, Ki Chang;Morisseau, Christophe;Gee, Shirley J.;Hammock, Bruce D.
抗菌肥皂添加剂三氯卡班（TCC）广泛应用于个人护理产品中。TCC具有较高的环境持久性。我们开发并验证了一种灵敏的在线固相萃取- lc -MS/MS方法，以快速分析尿液和其他生物样品中的TCC及其主要代谢物，以评估人体暴露。我们用含0.6% TCC的商业肥皂淋浴后0-72小时测量了人类尿液浓度。肾脏清除的主要途径是n -葡萄糖醛酸酯排泄。估计吸收量为所用肥皂中70±15 mg TCC的0.6%。TCC n -葡糖苷尿浓度在受试者之间差异很大，连续每天使用肥皂导致稳定的排泄水平。为了评估这种暴露所产生的潜在生物学效应，我们在体外筛选了TCC对人类酶的抑制作用。我们证明TCC是酶可溶性环氧化物水解酶（sEH）的有效抑制剂，而TCC的主要代谢物缺乏强抑制活性。在初步的体内研究中，局部给药TCC与大鼠相似，但未能改变血浆中sEH活性的生物标志物。总体而言，本文描述的分析策略表明，使用TCC肥皂会导致暴露水平，需要进一步评估。
The antibacterial soap additive triclocarban (TCC) is widely used in personal care products. TCC has a high environmental persistence. We developed and validated a sensitive online solid phase extraction-LC-MS/MS method to rapidly analyze TCC and its major metabolites in urine and other biological samples to assess human exposure. We measured human urine concentrations 0–72 h after showering with a commercial bar soap containing 0.6% TCC. The major route of renal elimination was excretion as N-glucuronides. The absorption was estimated at 0.6% of the 70±15 mg TCC in the soap used. The TCC N-glucuronide urine concentration varied widely among the subjects and continuous daily use of the soap led to steady state levels of excretion. In order to assess potential biological effects arising from this exposure we screened TCC for the inhibition of human enzymes in vitro. We demonstrate that TCC is a potent inhibitor of the enzyme soluble epoxide hydrolase (sEH), whereas TCC′s major metabolites lack strong inhibitory activity. Topical administration of TCC at similar levels to rats in a preliminary in vivo study however failed to alter plasma biomarkers of sEH activity. Overall the analytical strategy described here revealed that use of TCC soap causes exposure levels that warrant further evaluation.
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影响因子：
11.4
作者：
Halden, RU;Paull, DH
通讯作者：
Paull, DH
影响因子：
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作者：
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通讯作者：
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作者：
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作者：
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影响因子：
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作者：
Coogan, Melinda A.;Edziyie, Regina E.;Venables, Barney J.
通讯作者：
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