Conserved structural motifs in the central pair complex of eukaryotic flagella.
Conserved structural motifs in the central pair complex of eukaryotic flagella.
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DOI：
10.1002/cm.21094
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发表时间：
2013-02
期刊：
影响因子：
2.9
通讯作者：
Nicastro, Daniela
中科院分区：
文献类型：
--
作者：
Carbajal-Gonzalez, Blanca I.;Heuser, Thomas;Fu, Xiaofeng;Lin, Jianfeng;Smith, Brandon W.;Mitchell, David R.;Nicastro, Daniela
纤毛和鞭毛是真核细胞保守的毛发状附属物，具有传感和运动产生细胞器的功能。运动是由成千上万需要精确调节的轴突动力驱动的。一个重要的运动调节因子是中央对复合体（CPC），许多CPC缺陷导致纤毛/鞭毛瘫痪。一些人类疾病，如静止纤毛综合征，显示CPC异常，但对CPC的详细三维结构和功能知之甚少。CPC位于典型的[9+2]纤毛/鞭毛的中心，由两个单线态微管组成，每个单线态微管都有一组相关的突起，向周围的九个双线态微管延伸。利用低温电子断层扫描结合亚层析成像平均技术，以迄今为止公布的最高分辨率对绿藻衣藻和海胆圆心藻的CPC三维结构进行了可视化和比较。尽管这些物种之间的进化距离较远，但它们的cpc表现出显著的结构守恒。我们确定了几个新的突出部分，包括那些形成难以捉摸的鞘的部分，并表明这座桥的结构比以前想象的要复杂得多。生物特异性差异包括衣藻中存在微管内部蛋白，而圆心菌中没有；高度连接的投影网络的总体轮廓不同，藻类形成圆形圆柱体，而海胆则更呈椭圆形。这些差异可能是对衣藻旋转CPC的机械要求的适应，相比之下，圆形中心菌的CPC具有固定的方向。
Cilia and flagella are conserved hair-like appendages of eukaryotic cells that function as sensing and motility generating organelles. Motility is driven by thousands of axonemal dyneins that require precise regulation. One essential motility regulator is the central pair complex (CPC) and many CPC defects cause paralysis of cilia/flagella. Several human diseases, such as immotile cilia syndrome, show CPC abnormalities, but little is known about the detailed three-dimensional structure and function of the CPC. The CPC is located in the center of typical [9+2] cilia/flagella and is composed of two singlet microtubules, each with a set of associated projections that extend toward the surrounding nine doublet microtubules. Using cryo-electron tomography coupled with subtomogram averaging, we visualized and compared the three-dimensional structures of the CPC in both the green alga Chlamydomonas and the sea urchin Strongylocentrotus at the highest resolution published to date. Despite the evolutionary distance between these species, their CPCs exhibit remarkable structural conservation. We identified several new projections, including those that form the elusive sheath, and show that the bridge has a more complex architecture than previously thought. Organism-specific differences include the presence of microtubule inner proteins in Chlamydomonas, but not Strongylocentrotus, and different overall outlines of the highly connected projection network, which forms a round-shaped cylinder in algae, but is more oval in sea urchin. These differences could be adaptations to the mechanical requirements of the rotating CPC in Chlamydomonas, compared to the Strongylocentrotus CPC which has a fixed orientation.
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DOI：
10.1083/jcb.200912009
发表时间：
2010-05-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
DiPetrillo CG;Smith EF
通讯作者：
Smith EF
DOI：
10.1083/jcb.98.1.229
发表时间：
1984-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Dutcher SK;Huang B;Luck DJ
通讯作者：
Luck DJ
影响因子：
3.3
作者：
Barber CF;Heuser T;Carbajal-González BI;Botchkarev VV Jr;Nicastro D
通讯作者：
Nicastro D
DOI：
10.1083/jcb.136.1.167
发表时间：
1997-01-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Habermacher G;Sale WS
通讯作者：
Sale WS
影响因子：
4
作者：
Branche, Carole;Kohl, Linda;Bastin, Philippe
通讯作者：
Bastin, Philippe