Scanning ion conductance microscopy for studying biological samples.
Scanning ion conductance microscopy for studying biological samples.
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DOI：
10.3390/s121114983
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发表时间：
2012-11-06
期刊：
影响因子：
--
通讯作者：
Dietzel ID
中科院分区：
文献类型：
--
作者：
Happel P;Thatenhorst D;Dietzel ID
扫描离子电导显微镜(SICM)是一种扫描探头技术，它利用了当充满电解液的玻璃微吸管靠近导电性较差的表面时，接触电阻增加的情况。由于可以在扫描探头尖端与样品物理接触之前监测到电阻的增加，因此该技术对于研究活细胞等精致样品的形貌特别有用。SICM已显示出其在各种应用中的潜力，如活细胞的高分辨率和长时间成像或确定细胞体积的局部变化。此外，SICM还与各种技术相结合，如荧光显微镜或膜片钳，以揭示有关蛋白质或蛋白质功能的局部信息。本文详细介绍了SICM的各种优点和缺陷，并概述了SICM在生物成像中的最新发展和应用。此外，我们还表明，原则上，SICM和离子选择性微电极的结合使人们能够监测活细胞周围的局部离子活动。
Scanning ion conductance microscopy (SICM) is a scanning probe technique that utilizes the increase in access resistance that occurs if an electrolyte filled glass micro-pipette is approached towards a poorly conducting surface. Since an increase in resistance can be monitored before the physical contact between scanning probe tip and sample, this technique is particularly useful to investigate the topography of delicate samples such as living cells. SICM has shown its potential in various applications such as high resolution and long-time imaging of living cells or the determination of local changes in cellular volume. Furthermore, SICM has been combined with various techniques such as fluorescence microscopy or patch clamping to reveal localized information about proteins or protein functions. This review details the various advantages and pitfalls of SICM and provides an overview of the recent developments and applications of SICM in biological imaging. Furthermore, we show that in principle, a combination of SICM and ion selective micro-electrodes enables one to monitor the local ion activity surrounding a living cell.
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影响因子：
17.1
作者：
Chen CC;Zhou Y;Baker LA
通讯作者：
Baker LA
DOI：
10.1016/j.bbrc.2005.06.203
发表时间：
2005-09-09
影响因子：
3.1
作者：
Duclohier, H
通讯作者：
Duclohier, H
影响因子：
3.5
作者：
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通讯作者：
Ascoli, C
影响因子：
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作者：
Gorelik, J;Zhang, YJ;Korchev, YE
通讯作者：
Korchev, YE
影响因子：
3.4
作者：
Bruckbauer, Andreas;James, Peter;Klenerman, David
通讯作者：
Klenerman, David