Utilizing clathrin triskelions as carriers for spatially controlled multi-protein display.
Utilizing clathrin triskelions as carriers for spatially controlled multi-protein display.
复制标题
利用网格蛋白 triskelions 作为空间控制多蛋白展示的载体。
DOI：
10.1016/j.biomaterials.2016.08.044
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发表时间：
2016-11
期刊：
影响因子：
14
通讯作者：
Nguyen, Juliane
中科院分区：
文献类型：
--
作者：
Deci, Michael B.;Ferguson, Scott W.;Liu, Maixian;Peterson, Damian C.;Koduvayur, Sujatha P.;Nguyen, Juliane
关键词：
The simultaneous and spatially controlled display of different proteins on nanocarriers is a desirable property not often achieved in practice. Here, we report the use of clathrin triskelions as a versatile platform for functional protein display. We hypothesized that site-specific molecular epitope recognition would allow for effective and ordered protein attachment to clathrin triskelions. Clathrin binding peptides (CBPs) were genetically fused to mCherry and green fluorescent protein (GFP), expressed, and loaded onto clathrin triskelions by site-specific binding. Attachment was confirmed by surface plasmon resonance. mCherry fusion proteins modified with various CBPs displayed binding affinities between 470 nM and 287 μM for the clathrin triskelions. Simultaneous attachment of GFP-Wbox and mCherry-Cbox fusion constructs to the clathrin terminal domain was verified by Förster resonance energy transfer. The circulating half-lives, area under the curve, and the terminal half-lives of GFP and mCherry were significantly increased when attached to clathrin triskelions. Clathrin triskelion technology is useful for the development of versatile and multifunctional carriers for spatially controlled protein or peptide display with tremendous potential in nanotechnology, drug delivery, vaccine development, and targeted therapeutic applications.
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影响因子：
3.4
作者：
Jin, AJ;Nossal, R
通讯作者：
Nossal, R
影响因子：
56.9
作者：
Dell'Angelica, EC;Klumperman, J;Bonifacino, JS
通讯作者：
Bonifacino, JS
影响因子：
64.8
作者：
UNGEWICKELL, E;BRANTON, D
通讯作者：
BRANTON, D
DOI：
10.1083/jcb.91.3.790
发表时间：
1981-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Crowther RA;Pearse BM
通讯作者：
Pearse BM
影响因子：
3.3
作者：
Collette, John R.;Chi, Richard J.;Lemmon, Sandra K.
通讯作者：
Lemmon, Sandra K.