Dynamic Changes in Bacterial Communities During Compost and Earthworm Treatment of Low-Grade Potassium Ore
Dynamic Changes in Bacterial Communities During Compost and Earthworm Treatment of Low-Grade Potassium Ore
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堆肥和蚯蚓处理低品位钾矿过程中细菌群落的动态变化
DOI：
10.1080/01490451.2012.757999
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发表时间：
2013-01
影响因子：
2.3
通讯作者：
Lian, Bin
中科院分区：
文献类型：
--
作者：
Liu, Dian-Feng;Wang, Bin;Lian, Bin
堆肥和堆肥处理都能有效降解钾石粉，细菌在钾石粉的生物降解过程中起着关键作用。为了解矿石生物转化过程中的优势菌群及其组成，采用变性梯度凝胶电泳（DGGE）技术，研究了矿石粉与有机废弃物共堆肥后，经蚯蚓处理后堆肥中的细菌群落结构。结果表明，在固态发酵的前期，细菌群落结构变化很快，而在发酵后期和发酵过程中，细菌群落结构相对稳定。堆肥前期优势菌种类变化较大，堆肥后期和堆肥处理过程中优势菌种类基本稳定。DGGE图谱中以条带12（可能为交替单胞菌属）和条带14（可能为厚壁菌门）为代表的两类细菌被发现在整个固态发酵期间是优势种。处理过程中未出现特殊的优势菌种。基于16SrRNA序列的系统发育研究表明，主要的13个条带来自变形菌门和厚壁菌门，表明这些门中的细菌在堆肥处理过程中发挥了重要作用。
Both composting and earthworm treatment can degrade potassium rock powders and bacteria play a key role during the bio-processings. To understand the dominant bacteria and bacterial profile in biological conversion of the ore including compost and earthworm treatment, the bacterial communities in the compost were studied by using the method of denaturing gradient gel electrophoresis (DGGE) after ore powder was co-composted with organic wastes and then treated with earthworms. Results showed bacterial community structure changed very quickly during the early stages of solid-state fermentation, but relatively stable in the later stages of fermentation and during earthworm treatment. The dominant species of bacteria largely varied in the earlier stage of composting, but they were stable in the latter stage and during earthworm treatment. Two classes of bacteria, represented by band 12 (likely Alteromonas) and band 14 (likely Firmicutes) in DGGE profile, were found to be dominant species over the entire solid-state fermentation period. No special dominant bacterial species appeared during earthworm treatment. Phylogenetic studies of the bacteria based on 16S rRNA sequences indicate that major 13 bands came from phyla Proteobacteria and Firmicutes, suggesting that bacteria in these phyla played an important role during the compost treatment.
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影响因子：
4.9
作者：
Basak, B. B.;Biswas, D. R.
通讯作者：
Biswas, D. R.
影响因子：
4.4
作者：
S. Peters;Stefanie Koschinsky;F. Schwieger;C. Tebbe
通讯作者：
S. Peters;Stefanie Koschinsky;F. Schwieger;C. Tebbe
影响因子：
11.4
作者：
Tuomela, M;Vikman, M;Itävaara, M
通讯作者：
Itävaara, M
DOI：
--
发表时间：
2009-08
期刊：
Ying yong sheng tai xue bao = The journal of applied ecology
影响因子：
--
作者：
Kaizhi Xie;Pei-zhi Xu;Fabao Zhang;Jiansheng Chen;Shuanghu Tang;Xu Huang;Chaozhan Yan;Wenjie Gu
通讯作者：
Kaizhi Xie;Pei-zhi Xu;Fabao Zhang;Jiansheng Chen;Shuanghu Tang;Xu Huang;Chaozhan Yan;Wenjie Gu
影响因子：
3.7
作者：
Liu D;Lian B;Wang B;Jiang G
通讯作者：
Jiang G