Integrating Transcriptomic and ChIP-Seq Reveals Important Regulatory Regions Modulating Gene Expression in Myometrium during Implantation in Pigs.
Integrating Transcriptomic and ChIP-Seq Reveals Important Regulatory Regions Modulating Gene Expression in Myometrium during Implantation in Pigs.
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DOI：
10.3390/biom13010045
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发表时间：
2022-12-26
期刊：
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
--
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The myometrium is the outer layer of the uterus. Its contraction and steroidogenic activities are required for embryo implantation. However, the molecular mechanisms underlying its functions remain unknown in pigs. The myometrium includes the inner circular muscle (CM) and the outer longitudinal muscle (LM) layers. In this study, we collected the CM and LM samples from the mesometrial side (named M) of the uterus on days 12 (pre-implantation stage) and 15 (implantation stage) of pregnancy and day 15 of the estrous cycle. The transcriptomic results revealed distinct differences between the uterine CM and LM layers in early pregnancy: the genes expressed in the LM layer were mainly related to contraction pathways, whereas the transcriptional signatures in the CM layer on day 15 of pregnancy were primarily involved in the immune response processes. Subsequent comparisons in the CM layer between pregnant and cyclic gilts show that the transcriptional signatures of the CM layer are implantation-dependent. Next, we investigated the genome-wide profiling of histone H3 lysine 27 acetylation (H3K27ac) and histone H3 lysine 4 trimethylation (H3K4me3) in pig uterine CM and LM layers. The genomic regions that had transcriptional activity and were associated with the expression of genes in the two layers were characterized. Taken together, the regulatory regions identified in the study may contribute to modulating the gene expression in pig uterine CM and LM layers during implantation.
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影响因子：
64.8
作者：
Heintzman, Nathaniel D.;Hon, Gary C.;Hawkins, R. David;Kheradpour, Pouya;Stark, Alexander;Harp, Lindsey F.;Ye, Zhen;Lee, Leonard K.;Stuart, Rhona K.;Ching, Christina W.;Ching, Keith A.;Antosiewicz-Bourget, Jessica E.;Liu, Hui;Zhang, Xinmin;Green, Roland D.;Lobanenkov, Victor V.;Stewart, Ron;Thomson, James A.;Crawford, Gregory E.;Kellis, Manolis;Ren, Bing
通讯作者：
Ren, Bing
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
2.9
作者：
Franczak, Anita;Wojciechowicz, Bartosz;Kolakowska, Justyna;Zglejc, Kamila;Kotwica, Genowefa
通讯作者：
Kotwica, Genowefa
DOI：
10.1074/jbc.m110.197202
发表时间：
2011-02-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chen Q;Zhang Y;Peng H;Lei L;Kuang H;Zhang L;Ning L;Cao Y;Duan E
通讯作者：
Duan E
精神分裂症的大脑 ADRA2A 和 ADRA2C 基因表达差异及表观遗传调控。抗精神病药物治疗的效果。
影响因子：
6.8
作者：
Brocos-Mosquera I;Miranda-Azpiazu P;Muguruza C;Corzo-Monje V;Morentin B;Meana JJ;Callado LF;Rivero G
通讯作者：
Rivero G