Screening for streptomycin resistance-conferring mutations in Mycobacterium tuberculosis clinical isolates from Poland.
Screening for streptomycin resistance-conferring mutations in Mycobacterium tuberculosis clinical isolates from Poland.
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DOI：
10.1371/journal.pone.0100078
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Bielecki J
中科院分区：
文献类型：
--
作者：
Jagielski T;Ignatowska H;Bakuła Z;Dziewit Ł;Napiórkowska A;Augustynowicz-Kopeć E;Zwolska Z;Bielecki J
目前，三个基因（分别编码16S rRNA、核糖体蛋白S12和16S rRNA特异性甲基转移酶的rrs、rpsL和gidB）的突变被认为与结核分枝杆菌对链霉素（STR）的耐药性有关。本研究的目的是调查结核分枝杆菌临床分离株（对 STR 具有耐药性和敏感性）中这些突变的谱和频率。该研究纳入了 2004 年从波兰结核病患者体内回收的 64 株结核分枝杆菌分离株。样本中有 50 株多重耐药菌株（32 株 STR 耐药，18 株 STR 敏感）和 14 株泛敏感菌株。采用1%比例法进行STR抗性初步检测。通过Etest方法测定STR的MIC。通过对整个 rrs、rpsL 和 gidB 基因进行扩增和测序来进行突变分析。在 23 株（71.9%）STR 耐药菌株中检测到 rrs 或 rpsL 基因的非同义突变，而没有 STR 敏感菌株检测到。 gidB 基因突变分布在 12 个 (37.5%) STR 抗性菌株和 13 个 (40.6%) STR 敏感菌株中。四个 (12.5%) STR 抗性分离株在所有三个检查位点均为野生型。 rrs、rpsL 或 gidB 突变均不能与低、中或高水平的 STR 抗性相关。根据之前的研究结果，gidB 47T→G（L16R）突变与拉丁美洲-地中海基因型家族相关，而gidB基因的276A→C（E92D）和615A→G（A205A）突变与北京谱系相关。该研究强调了 rrs 和 rpsL 突变作为 STR 抗性分子标记的有用性，但并未表明其水平。 gidB 多态性可以作为系统发育标记。
Currently, mutations in three genes, namely rrs, rpsL, and gidB, encoding 16S rRNA, ribosomal protein S12, and 16S rRNA-specific methyltransferase, respectively, are considered to be involved in conferring resistance to streptomycin (STR) in Mycobacterium tuberculosis. The aim of this study was to investigate the spectrum and frequency of these mutations in M. tuberculosis clinical isolates, both resistant and susceptible to STR. Sixty-four M. tuberculosis isolates recovered from as many TB patients from Poland in 2004 were included in the study. Within the sample were 50 multidrug-resistant (32 STR-resistant and 18 STR-susceptible) and 14 pan-susceptible isolates. Preliminary testing for STR resistance was performed with the 1% proportion method. The MICs of STR were determined by the Etest method. Mutation profiling was carried out by amplifying and sequencing the entire rrs, rpsL, and gidB genes. Non-synonymous mutations in either rrs or rpsL gene were detected in 23 (71.9%) of the STR-resistant and none of the STR-susceptible isolates. Mutations in the gidB gene were distributed among 12 (37.5%) STR-resistant and 13 (40.6%) STR-susceptible isolates. Four (12.5%) STR-resistant isolates were wild-type at all three loci examined. None of the rrs, rpsL or gidB mutations could be linked to low, intermediate or high level of STR resistance. In accordance with previous findings, the gidB 47T→G (L16R) mutation was associated with the Latin American-Mediterranean genotype family, whereas 276A→C (E92D) and 615A→G (A205A) mutations of the gidB gene were associated with the Beijing lineage. The study underlines the usefulness of rrs and rpsL mutations as molecular markers for STR resistance yet not indicative of its level. The gidB polymorphisms can serve as phylogenetic markers.
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影响因子：
9.4
作者：
Spies, Fernanda S.;Ribeiro, Andrezza W.;Zaha, Arnaldo
通讯作者：
Zaha, Arnaldo
影响因子：
3.3
作者：
Dobner, P;Bretzel, G;Rinder, H
通讯作者：
Rinder, H
影响因子：
4.2
作者：
Ballif M;Harino P;Ley S;Coscolla M;Niemann S;Carter R;Coulter C;Borrell S;Siba P;Phuanukoonnon S;Gagneux S;Beck HP
通讯作者：
Beck HP
影响因子：
3.7
作者：
Homolka S;Projahn M;Feuerriegel S;Ubben T;Diel R;Nübel U;Niemann S
通讯作者：
Niemann S
影响因子：
4.9
作者：
Siddiqi, N;Shamim, M;Hasnain, SE
通讯作者：
Hasnain, SE