A novel lytic phage potentially effective for phage therapy against Burkholderia pseudomallei in the tropics.
A novel lytic phage potentially effective for phage therapy against Burkholderia pseudomallei in the tropics.
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一种新的裂解性噬菌体，对热带地区的类鼻疽伯克霍尔德菌的噬菌体治疗可能有效。
DOI：
10.1186/s40249-022-01012-9
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发表时间：
2022-08-04
影响因子：
8.1
通讯作者：
中科院分区：
文献类型：
--
作者：
类鼻疽伯克霍尔德菌是一种热带病原体，可引起类鼻疽病。其内在的耐药性是治疗失败的主要原因，并且少数可用的抗生素需要长期使用才能有效。本研究旨在评估B的临床潜力。从海南分离到的类鼻疽杆菌。 以类鼻疽伯克霍尔德菌（HNBP 001）为分离宿主，从国内环境中回收病原菌，进行宿主范围测定、裂解特性测定和稳定性试验。通过透射电子显微镜检查最佳候选物以进行分类。通过对其基因组的测序和分析，研究了其对B.评估A549细胞和秀丽隐杆线虫中的类鼻疽感染，其中使用单因素ANOVA方法和对数秩检验比较细胞活力和存活率。回收了能够裂解24/25个临床分离株的噬菌体。它被归类为短尾病毒科，并被发现适合繁殖。在最佳感染复数（MOI）为0.1的条件下，噬菌期约为20 min，在1 h内产生高滴度（1012 PFU/ml）。发现该噬菌体在宽范围的温度（24、37、40、50和60 °C）和pH值（3-12）下稳定。命名为vB_BpP_HN01后，对其进行了全序列测定，其71，398 bp的线性基因组包含93个开放阅读框和一个tRNA-Asn，与已知病毒的序列相似性较低。此外，发现单独或与抗生素组合应用vB_BpP_HN01（MOI = 0.1和MOI = 1）的保护作用提高了感染细胞的存活率（分别为70.6 ± 6.8%、85.8 ± 5.7%、91.9 ± 1.8%和96.8 ± 1.8%）。在感染的C. elegans后添加此噬菌体。作为第一个B。从中国海南分离到一株类鼻疽杆菌噬菌体vB_BpP_HN01，其特征在于在体外/体内实验中具有良好的溶解特性、稳定性和细菌清除效率。因此，我们可以得出结论，它是一种潜在的替代药物，用于打击类鼻疽。在线版本包含补充材料，可通过10.1186/s40249-022-01012-9获得。
Burkholderia pseudomallei is a tropical pathogen that causes melioidosis. Its intrinsic drug-resistance is a leading cause of treatment failure, and the few available antibiotics require prolonged use to be effective. This study aimed to assess the clinical potential of B. pseudomallei phages isolated from Hainan, China. Burkholderia pseudomallei strain (HNBP001) was used as the isolation host, and phages were recovered from domestic environmental sources, which were submitted to the host range determination, lytic property assays, and stability tests. The best candidate was examined via the transmission electron microscope for classification. With its genome sequenced and analyzed, its protective efficacy against B. pseudomallei infection in A549 cells and Caenorhabditis elegans was evaluated, in which cell viability and survival rates were compared using the one-way ANOVA method and the log-rank test. A phage able to lyse 24/25 clinical isolates was recovered. It was classified in the Podoviridae family and was found to be amenable to propagation. Under the optimal multiplicity of infection (MOI) of 0.1, an eclipse period of around 20 min and a high titer (1012 PFU/ml) produced within 1 h were demonstrated. This phage was found stabile at a wide range of temperatures (24, 37, 40, 50, and 60 °C) and pH values (3–12). After being designated as vB_BpP_HN01, it was fully sequenced, and the 71,398 bp linear genome, containing 93 open reading frames and a tRNA-Asn, displayed a low sequence similarity with known viruses. Additionally, protective effects of applications of vB_BpP_HN01 (MOI = 0.1 and MOI = 1) alone or in combination with antibiotics were found to improve viability of infected cells (70.6 ± 6.8%, 85.8 ± 5.7%, 91.9 ± 1.8%, and 96.8 ± 1.8%, respectively). A significantly reduced mortality (10%) and a decreased pathogen load were demonstrated in infected C. elegans following the addition of this phage. As the first B. pseudomallei phage was isolated in Hainan, China, phage vB_BpP_HN01 was characterized by promising lytic property, stability, and efficiency of bacterial elimination during the in vitro/vivo experiments. Therefore, we can conclude that it is a potential alternative agent for combating melioidosis. The online version contains supplementary material available at 10.1186/s40249-022-01012-9.
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影响因子：
4.6
作者：
Garneau JR;Depardieu F;Fortier LC;Bikard D;Monot M
通讯作者：
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4.9
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影响因子：
14.9
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3.7
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