Amylase-binding proteins A (AbpA) and B (AbpB) differentially affect colonization of rats' teeth by Streptococcus gordonii.
Amylase-binding proteins A (AbpA) and B (AbpB) differentially affect colonization of rats' teeth by Streptococcus gordonii.
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淀粉酶结合蛋白 A (AbpA) 和 B (AbpB) 对戈登链球菌在大鼠牙齿上的定植有不同的影响。
DOI：
10.1099/mic.0.26022-0
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Scannapieco,FA
中科院分区：
文献类型：
--
作者：
Tanzer,JM;Grant,L;Thompson,A;Li,L;Rogers,JD;Haase,EM;Scannapieco,FA
戈登链球菌产生两种α-淀粉酶结合蛋白，abpA和abpB，已在体外进行了广泛的研究。然而，人们对它们在口腔定植和致龋性(毒力)中的意义知之甚少。为了阐明这些问题，将断奶的无特定病原体的奥斯本-孟德尔大鼠Tan ： SPFOM(OM)BR接种野生型FAS4-S或Challis-S菌株，或接种具有abpA、abpB等基因突变的菌株，或同时接种两者，以比较它们在牙齿上的定植能力和持久性。实验是在喂食高蔗糖食物的大鼠身上进行的，食物中含有少量淀粉或只含有淀粉。对突变体和野生型进行活体定量，并对龋损进行评分。在11个实验中，当给老鼠喂食通常占主导地位的蔗糖(低淀粉)饲料时，戈登氏葡萄球菌是牙齿的多菌落。无论哪种接种剂类型，喂食蔗糖的大鼠的接种剂回收率都比那些食用不含蔗糖、添加淀粉的饮食的大鼠高出数倍。AbpB缺陷菌株不能在只吃淀粉的大鼠的牙齿上定植，但如果在饮食中添加蔗糖，则可以定植在大鼠的牙齿上。令人惊讶的是，ABPA缺陷菌株的定殖率比它们的野生型要好得多。同时缺乏ABPA和ABB的双重突变体(ABPA/ABPB)与其野生型一样定殖。野生型FAS4-S和Challis-S仅具有边缘致龋性。值得注意的是，在没有ABPA的情况下，致龋性略有增加。AbpB−突变体对侵染的挽救和abpa−突变体在饲料蔗糖存在下对侵染的增强都表明了与侵袭有关的额外的淀粉酶结合蛋白相互作用。糖基转移酶活性在abpA缺陷突变体中较高，在abpB突变体中略有升高。结果表明，AbpB是摄食淀粉的大鼠牙齿定植所必需的，如果大鼠食用蔗糖-淀粉饮食，AbpB的缺失可以部分被掩盖。ABPA似乎能抑制体内菌斑生物膜的定植。这种意想不到的活体效应可能与ABPA与葡萄糖转移酶的相互作用或与这些细胞的其他定植因素有关。这些数据表明，口腔环境的复杂性可能不能在体外系统中得到充分的模拟。
Streptococcus gordoniiproduces twoα-amylase-binding proteins, AbpA and AbpB, that have been extensively studiedin vitro. Little is known, however, about their significance in oral colonization and cariogenicity (virulence). To clarify these issues, weanling specific pathogen-free Osborne-Mendel rats, TAN : SPFOM(OM)BR, were inoculated either with wild-type strains FAS4-S or Challis-S or with strains having isogenic mutations ofabpA,abpB, or both, to compare their colonization abilities and persistence on the teeth. Experiments were done with rats fed a sucrose-rich diet containing low amounts of starch or containing only starch. The mutants and wild-types were quantifiedin vivoand carious lesions were scored. In 11 experiments,S. gordoniiwas a prolific colonizer of the teeth when rats were fed the sucrose (with low starch)-supplemented diet, often dominating the flora. Sucrose-fed rats had several-fold higher recoveries of inoculants than those eating the sucrose-free, starch-supplemented diet, regardless of inoculant type. The strain defective in AbpB could not colonize teeth of starch-only-eating rats, but could colonize rats if sucrose was added to the diet. Strains defective in AbpA surprisingly colonized better than their wild-types. A double mutant deficient in both AbpA and AbpB (abpA/abpB) colonized like its wild-type. Wild-types FAS4-S and Challis-S had no more than marginal cariogenicity. Notably, in the absence of AbpA, cariogenicity was slightly augmented. Both the rescue of colonization by the AbpB−mutant and the augmentation of colonization by AbpA−mutant in the presence of dietary sucrose suggested additional amylase-binding protein interactions relevant to colonization. Glucosyltransferase activity was greater in mutants defective inabpAand modestly increased in theabpBmutant. It was concluded that AbpB is required for colonization of teeth of starch-eating rats and its deletion is partially masked if rats eat a sucrose-starch diet. AbpA appears to inhibit colonization of the plaque biofilmin vivo. This unexpected effectin vivomay be associated with interaction of AbpA with glucosyltransferase or with other colonization factors of these cells. These data illustrate that the complex nature of the oral environment may not be adequately modelled byin vitrosystems.
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口腔链球菌淀粉酶结合蛋白的比较。
影响因子：
2.1
作者：
J. Gwynn;C. Douglas
通讯作者：
C. Douglas
口腔链球菌及其粘附缺陷突变体在唾液涂层羟基磷灰石上的蔗糖依赖性积累。
影响因子：
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作者：
M. Vickerman;G. Jones
通讯作者：
G. Jones
人唾液α-淀粉酶的酶促和链球菌结合位点之间的结构关系。
DOI：
10.1016/s0006-291x(05)80900-3
发表时间：
1990
影响因子：
3.1
作者：
Scannapieco,FA;Bhandary,K;Ramasubbu,N;Levine,MJ
通讯作者：
Levine,MJ
大鼠口腔与颊舌、近端及沟沟龋的统计关系
影响因子：
7.6
作者：
L. A. Rinehimer;J. Tanzer
通讯作者：
J. Tanzer
使用复制板测定快速评估唾液蛋白-细菌相互作用。
影响因子：
--
作者：
Tseng,CC;Scannapieco,FA;Levine,MJ
通讯作者：
Levine,MJ