Microporation and 'iron'tophoresis for treating iron deficiency anemia.
Microporation and 'iron'tophoresis for treating iron deficiency anemia.
复制标题
DOI：
10.1007/s11095-012-0930-2
复制
发表时间：
2013-03
影响因子：
3.7
通讯作者：
Murthy, S. Narasimha
中科院分区：
文献类型：
--
作者：
Modepalli, Naresh;Jo, Seongbong;Repka, Michael A.;Murthy, S. Narasimha
关键词：
研究了焦磷酸铁（FPP）离子导入结合微针预处理治疗缺铁性贫血（IDA）的可能性。使用无毛大鼠皮肤进行体外透皮给药研究，并在无毛贫血大鼠模型中进行药效学研究。在健康、贫血状态和治疗后监测大鼠的血液学和生化参数，如血红蛋白、红细胞压积和%血清转铁蛋白。在用苏木精和伊红染色后，在组织学皮肤切片中观察由微针产生的微孔。通过在不同时间点测量经表皮水分损失（TEWL）来在体内研究微孔的恢复。当单独使用时，被动、微针和离子电渗介导的递送没有导致贫血大鼠血液学和生化参数的显著改善。当电离子导入（0.15mA/cm ~ 2，4 h）与微针预处理（2 min）相结合时，递送了治疗上足够量的FPP，并且大鼠从IDA中有显著的恢复。经皮穿刺针和离子导入介导的铁递送可作为IDA的潜在治疗方法。经皮控释铁剂可能成为一种潜在的、安全的、有效的替代肠外铁剂治疗的药物。
Iontophoretic mediated transdermal delivery of ferric pyrophosphate (FPP) in combination with microneedle pretreatment was investigated as a potential treatment for iron deficiency anemia (IDA). In vitro transdermal delivery studies were performed using hairless rat skin and pharmacodynamic studies were performed in hairless anemic rat model. The hematological and biochemical parameters like hemoglobin, hematocrit and % serum transferrin were monitored in rats at healthy, anemic condition and post treatment. Micropores created by the microneedles were visualized in histological skin sections after staining with hemotoxylin and eosin. The recovery of micropores was investigated in vivo by measuring Transepidermal water loss (TEWL) at different time points. The passive, microneedle and iontophoresis mediated delivery did not lead to significant improvement in hematological and biochemical parameters in anemic rats, when used individually. When iontophoresis (0.15 mA/cm2 for 4 hours) was combined with microneedle pretreatment (for 2 minutes), therapeutically adequate amount of FPP was delivered and there was significant recovery of rats from IDA. Microneedle and iontophoresis mediated delivery of iron via transdermal route could be developed as a potential treatment for IDA. The transdermal controlled delivery of iron could become a potential, safe and effective alternative to parenteral iron therapy.
登录
查看更多内容
影响因子：
4.2
作者：
Johnson-Wimbley, Terri D;Graham, David Y
通讯作者：
Graham, David Y
影响因子：
3.8
作者：
Murthy, S. Narasimha;Vaka, Siva Ram Kiran
通讯作者：
Vaka, Siva Ram Kiran
影响因子：
3.7
作者：
Kalluri, Haripriya;Banga, Ajay K.
通讯作者：
Banga, Ajay K.
影响因子：
29.4
作者：
Forbes, Alastair
通讯作者：
Forbes, Alastair
影响因子：
19.6
作者：
Gupta, A;Amin, NB;Anandan, JV
通讯作者：
Anandan, JV