Loss of extracellular matrix from articular cartilage is mediated by the synovium and ligament after anterior cruciate ligament injury.
Loss of extracellular matrix from articular cartilage is mediated by the synovium and ligament after anterior cruciate ligament injury.
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DOI：
10.1016/j.joca.2013.09.003
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发表时间：
2013-12
影响因子：
7
通讯作者：
Murray, M. M.
中科院分区：
文献类型：
--
作者：
Haslauer, C. M.;Elsaid, K. A.;Fleming, B. C.;Proffen, B. L.;Johnson, V. M.;Murray, M. M.
创伤后骨关节炎（PTOA）发生在前交叉韧带（ACL）损伤后。PTOA可能是由能够在损伤时引起关节软骨降解的蛋白水解酶的早期表达引发的。本研究调查了ACL损伤后多个关节组织中三种关键蛋白酶的产生以及随后的软骨周转标志物。在青少年小型猪中进行ACL横断。在损伤后第1、5、9和14天，量化关节软骨、滑膜、损伤韧带和临时支架中胶原酶（MMP-1和MMP-13）和聚集蛋白聚糖酶（ADAMTS-4）基因表达变化。在血清和滑液中定量胶原降解（C2C）、合成（CPII）和聚集蛋白聚糖合成（CS 846）的标志物。还进行了软骨完整性的组织学评估（OARSI评分）。ACL损伤后关节软骨、滑膜和韧带中MMP-1基因表达上调。MMP-13在关节软骨中表达受到抑制，但在滑膜和韧带中上调100倍。ADAMTS-4在滑膜和韧带中上调，但在关节软骨中不上调。在损伤后的前五天，滑膜关节液中胶原降解片段（C2C）的浓度几乎翻了一番。我们得出结论，编码能够降解软骨ECM的蛋白质的基因的上调是在ACL损伤后的头几天内看到的，并且这种反应不仅在软骨细胞中看到，而且在滑膜、韧带和临时支架中的细胞中看到。
Post-traumatic osteoarthritis (PTOA) occurs after anterior cruciate ligament (ACL) injury. PTOA may be initiated by early expression of proteolytic enzymes capable of causing degradation of the articular cartilage at time of injury. This study investigated the production of three of these key proteases in multiple joint tissues after ACL injury and subsequent markers of cartilage turnover. ACL transection was performed in adolescent minipigs. Collagenase (MMP-1 and MMP-13) and aggrecanase (ADAMTS-4) gene expression changes were quantified in the articular cartilage, synovium, injured ligament, and the provisional scaffold at days 1, 5, 9, and 14 post-injury. Markers of collagen degradation (C2C), synthesis (CPII) and aggrecan synthesis (CS846) were quantified in the serum and synovial fluid. Histologic assessment of the cartilage integrity (OARSI scoring) was also performed. MMP-1 gene expression was upregulated in the articular cartilage, synovium and ligament after ACL injury. MMP-13 expression was suppressed in the articular cartilage, but upregulated 100fold in the synovium and ligament. ADAMTS-4 was upregulated in the synovium and ligament but not in the articular cartilage. The concentration of collagen degradation fragments (C2C) in the synovial joint fluid nearly doubled in the first five days after injury. We conclude that upregulation of genes coding for proteins capable of degrading cartilage ECM is seen within the first few days after ACL injury, and this response is seen not only in chondrocytes, but also in cells in the synovium, ligament and provisional scaffold.
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影响因子：
2.8
作者：
Chamberlain, Connie S.;Crowley, Erin M.;Kobayashi, Hirohito;Eliceiri, Kevin W.;Vanderby, Ray
通讯作者：
Vanderby, Ray
影响因子：
--
作者：
Jay, Gregory D.;Fleming, Braden C.;Watkins, Bryn A.;McHugh, Karen A.;Anderson, Scott C.;Zhang, Ling X.;Teeple, Erin;Waller, Kimberly A.;Elsaid, Khaled A.
通讯作者：
Elsaid, Khaled A.
影响因子：
15.9
作者：
Nelson, F;Dahlberg, L;Poole, AR
通讯作者：
Poole, AR
DOI：
10.1016/s0304-4165(01)00122-2
发表时间：
2001-05-03
影响因子：
3
作者：
Bluteau, G;Conrozier, T;Mallein-Gerin, F
通讯作者：
Mallein-Gerin, F
DOI：
10.1097/01.blo.0000197079.26600.09
发表时间：
2006-01-01
影响因子：
4.2
作者：
Andriacchi, Thomas P.;Briant, Paul L.;Koo, Seungbum
通讯作者：
Koo, Seungbum