Loss-of-function of Constitutive Expresser of Pathogenesis Related Genes5 affects potassium homeostasis in Arabidopsis thaliana.
Loss-of-function of Constitutive Expresser of Pathogenesis Related Genes5 affects potassium homeostasis in Arabidopsis thaliana.
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DOI：
10.1371/journal.pone.0026360
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Salt DE
中科院分区：
文献类型：
--
作者：
Borghi M;Rus A;Salt DE
在此，我们证明，先前在快中子诱变拟南芥离子组学筛选中发现的突变体中观察到的叶片 K+ 减少是由 CPR5 功能丧失等位基因引起的，我们将其命名为 cpr5-3。这一观察结果将低叶 K+ 确立为 CPR5 功能丧失等位基因的新表型。我们利用水杨酸 (SA) 和茉莉酸 (JA) 信号传导缺陷的双突变体，并通过各种通道和转运蛋白的基因表达分析，研究了影响 cpr5 低叶 K+ 的因素。 cpr5 和 Col-0 之间的相互嫁接用于确定芽和根在导致 cpr5 低叶 K+ 表型中的相对重要性。我们的数据表明，芽中 CPR5 功能的丧失主要决定了 cpr5 的低叶 K+ 表型，尽管根也有较小程度的影响。 cpr5 的低叶 K+ 表型与 cpr5 中已知的 SA 和 JA 升高无关。在 cpr5 中，编码各种环核苷酸门控通道 (CNGC) 的基因表达在叶子中独特地升高。此外，编码高亲和力 K+ 摄取转运蛋白的 HAK5 的表达在高或低 K+ 供应下生长的 cpr5 根中减少。我们提出了一个模型，其中 cpr5 中的低叶 K+ 主要是由各种 CNGC 表达的组成型激活引起的从茎到根的 K+ 输出增强驱动的。这种激活可以通过增强胞质 Ca2+ 间接增强 K+ 流出，和/或通过增加 K+ 转运活性直接增强 K+ 流出。增强的茎至根 K+ 输出也可能导致 cpr5 根中观察到的 HAK5 表达减少，从而导致 K+ 吸收减少。所有提供的离子组学数据均可在 www.ionomicshub.org 上公开获取。
Here, we demonstrate that the reduction in leaf K+ observed in a mutant previously identified in an ionomic screen of fast neutron mutagenized Arabidopsis thaliana is caused by a loss-of-function allele of CPR5, which we name cpr5-3. This observation establishes low leaf K+ as a new phenotype for loss-of-function alleles of CPR5. We investigate the factors affecting this low leaf K+ in cpr5 using double mutants defective in salicylic acid (SA) and jasmonic acid (JA) signalling, and by gene expression analysis of various channels and transporters. Reciprocal grafting between cpr5 and Col-0 was used to determine the relative importance of the shoot and root in causing the low leaf K+ phenotype of cpr5. Our data show that loss-of-function of CPR5 in shoots primarily determines the low leaf K+ phenotype of cpr5, though the roots also contribute to a lesser degree. The low leaf K+ phenotype of cpr5 is independent of the elevated SA and JA known to occur in cpr5. In cpr5 expression of genes encoding various Cyclic Nucleotide Gated Channels (CNGCs) are uniquely elevated in leaves. Further, expression of HAK5, encoding the high affinity K+ uptake transporter, is reduced in roots of cpr5 grown with high or low K+ supply. We suggest a model in which low leaf K+ in cpr5 is driven primarily by enhanced shoot-to-root K+ export caused by a constitutive activation of the expression of various CNGCs. This activation may enhance K+ efflux, either indirectly via enhanced cytosolic Ca2+ and/or directly by increased K+ transport activity. Enhanced shoot-to-root K+ export may also cause the reduced expression of HAK5 observed in roots of cpr5, leading to a reduction in uptake of K+. All ionomic data presented is publically available at www.ionomicshub.org.
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影响因子：
6.7
作者：
García AV;Blanvillain-Baufumé S;Huibers RP;Wiermer M;Li G;Gobbato E;Rietz S;Parker JE
通讯作者：
Parker JE
影响因子：
3.5
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影响因子：
5.3
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