Sex-specific 24-h profile of extracellular serotonin levels in the medial prefrontal cortex
Sex-specific 24-h profile of extracellular serotonin levels in the medial prefrontal cortex
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内侧前额叶皮层细胞外血清素水平的性别特异性 24 小时概况
DOI：
10.1016/j.brainres.2008.12.084
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发表时间：
2009
期刊：
影响因子：
2.9
通讯作者：
D. Mitsushima
中科院分区：
文献类型：
--
作者：
S. Jitsuki;F. Kimura;T. Funabashi;Takuya Takahashi;D. Mitsushima
内侧前额叶皮质（mPFC）控制许多物种的情绪反应，接受来自中缝背核和中缝核（DRN和MRN）的多巴胺能神经支配。为了研究mPFC细胞外5-羟色胺（5-HT）水平的24小时曲线的性别差异，使用完整的雄性、动情间期雌性和动情前期雌性大鼠进行了体内微透析研究。在自由移动条件下，每30分钟通过微透析探针从mPFC自动收集透析液超过24小时。通过高效液相色谱法定量透析液中的5 HT水平。细胞外5-HT水平表现出偶发性的变化，在mPFC的两种性别的大鼠，与两个diestrous和proestrous女性表现出明确的昼夜变化，5-HT水平高，在黑暗阶段，但在光相低。相反，雄性大鼠全天表现出相对较高的5-HT水平，没有明显的昼夜变化。在数学分析谷，男性表现出较高的5 HT水平比发情间期或发情前期的女性。总体24小时5-HT水平在男性显着大于动情前期的女性，但没有不同的动情间期的女性。此外，体视学方法被用来检查色氨酸羟化酶（TrpH）的数量，但没有性别差异的TrpH免疫反应细胞的数量在DRN和MRN观察。这些结果表明，性别和/或性腺类固醇环境可能会影响24小时的配置文件的细胞外5 HT在大鼠mPFC的DRN和MRN的5 HT神经元的数量没有变化。
The medial prefrontal cortex (mPFC) controls emotional responses in many species, receiving serotonergic innervation from the dorsal and median raphe nucleus (DRN and MRN). To examine the sex difference in 24-h profiles of extracellular serotonin (5HT) levels in the mPFC, an in vivo microdialysis study was performed using intact male, diestrous female, and proestrous female rats. Dialysates were automatically collected by a microdialysis probe from the mPFC every 30 min for more than 24 h under freely moving conditions. The levels of 5HT in dialysates were quantified by high performance liquid chromatography. Extracellular 5HT levels exhibited episodic changes in the mPFC of both sexes of rats, with both diestrous and proestrous females exhibiting a clear diurnal change; the 5HT levels were high during the dark phase, but low during the light phase. In contrast, male rats exhibited relatively high 5HT levels throughout the day without significant diurnal changes. At mathematically analyzed trough, males showed higher 5HT levels than diestrous or proestrous females. The overall 24-h 5HT levels in males were significantly greater than proestrous females, but were not different from diestrous females. Further, stereological methods were used to examine the number of tryptophan hydroxylase (TrpH), but no sex differences in the number of TrpH immunoreactive cells in the DRN and MRN were observed. These results suggest that sex and/or the gonadal steroid environment may affect the 24-h profile of extracellular 5HT in the mPFC of rats without changes in the number of 5HT neurons in the DRN and MRN.
影响因子：
56.9
作者：
DAMASIO, H;GRABOWSKI, T;DAMASIO, AR
通讯作者：
DAMASIO, AR
影响因子：
6.6
作者：
Kessler, RC
通讯作者：
Kessler, RC
DOI：
10.1073/pnas.94.10.5308
发表时间：
1997-05-13
影响因子：
11.1
作者：
Nishizawa, S;Benkelfat, C;Diksic, M
通讯作者：
Diksic, M