Ultrastructural modifications induced by SARS-CoV-2 in Vero cells: a kinetic analysis of viral factory formation, viral particle morphogenesis and virion release.
Ultrastructural modifications induced by SARS-CoV-2 in Vero cells: a kinetic analysis of viral factory formation, viral particle morphogenesis and virion release.
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DOI：
10.1007/s00018-020-03745-y
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发表时间：
2021-04
期刊：
影响因子：
--
通讯作者：
Roingeard P
中科院分区：
文献类型：
--
作者：
Eymieux S;Rouillé Y;Terrier O;Seron K;Blanchard E;Rosa-Calatrava M;Dubuisson J;Belouzard S;Roingeard P
目前对SARS-CoV-2的研究主要集中在短时间内，但很少有人关注感染细胞的超微结构特征。我们用透射电镜对SARS-CoV-2感染细胞的超微结构进行了动态分析。早期感染事件的特征在于存在单膜囊泡簇和含有核孔的膜堆叠，称为环状层（AL）。随后在细胞中观察到宿主细胞衍生的细胞器转化为病毒工厂的大型网络。正如之前对其他RNA病毒的描述，这些复制工厂由位于细胞核附近的双膜囊泡（DMV）组成。通过胞吐作用在细胞表面释放的病毒具有典型的刺突蛋白的冠，但在细胞内隔室中也观察到没有刺突的病毒颗粒，这可能反映了不正确的组装或细胞降解过程。
Many studies on SARS-CoV-2 have been performed over short-time scale, but few have focused on the ultrastructural characteristics of infected cells. We used TEM to perform kinetic analysis of the ultrastructure of SARS-CoV-2-infected cells. Early infection events were characterized by the presence of clusters of single-membrane vesicles and stacks of membrane containing nuclear pores called annulate lamellae (AL). A large network of host cell-derived organelles transformed into virus factories was subsequently observed in the cells. As previously described for other RNA viruses, these replication factories consisted of double-membrane vesicles (DMVs) located close to the nucleus. Viruses released at the cell surface by exocytosis harbored the typical crown of spike proteins, but viral particles without spikes were also observed in intracellular compartments, possibly reflecting incorrect assembly or a cell degradation process.
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DOI：
10.1007/s00018-012-1213-0
发表时间：
2013-04
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Ferraris P;Beaumont E;Uzbekov R;Brand D;Gaillard J;Blanchard E;Roingeard P
通讯作者：
Roingeard P
影响因子：
6.7
作者：
Neufeldt CJ;Joyce MA;Levin A;Steenbergen RH;Pang D;Shields J;Tyrrell DL;Wozniak RW
通讯作者：
Wozniak RW
影响因子：
12.7
作者：
Ng, ML;Tan, SH;Ling, AE
通讯作者：
Ling, AE
DOI：
10.1099/jgv.0.001453
发表时间：
2020-09
期刊：
The Journal of general virology
影响因子：
--
作者：
Ogando NS;Dalebout TJ;Zevenhoven-Dobbe JC;Limpens RWAL;van der Meer Y;Caly L;Druce J;de Vries JJC;Kikkert M;Bárcena M;Sidorov I;Snijder EJ
通讯作者：
Snijder EJ
影响因子：
2.6
作者：
Marshall, JA;Borg, J;Anderson, DA
通讯作者：
Anderson, DA