Novel peptides derived from egg white protein inhibiting alpha-glucosidase
Novel peptides derived from egg white protein inhibiting alpha-glucosidase
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源自蛋清蛋白的新型肽抑制α-葡萄糖苷酶
DOI：
10.1016/j.foodchem.2011.05.067
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发表时间：
2011-12
期刊：
影响因子：
8.8
通讯作者：
Chen, Feng
中科院分区：
文献类型：
--
作者：
Yu, Zhipeng;Yin, Yongguang;Zhao, Wenzhu;Yu, Yiding;Liu, Boqun;Liu, Jingbo;Chen, Feng
本研究的目的是寻找和鉴定具有α-葡萄糖苷酶和α-淀粉酶抑制活性的潜在抗糖尿病肽。Alcalase水解后，纯化蛋清蛋白水解产物，采用LC-MS-MS进行鉴定。用Fmoc固相合成法进一步合成鉴定出的8个多肽。通过α-葡萄糖苷酶和α-淀粉酶的酶抑制实验，评价了合成肽的抗糖尿病活性。其中，RVPSLM肽是潜在的α-葡萄糖苷酶抑制剂，ic50值为23.07μmolL−1。然而，它对α-淀粉酶没有明显的或可检测的抑制效率。这些研究表明，将蛋清蛋白水解物作为一种具有抗糖尿病活性的功能性食品具有潜力。
The aim of this study was to search and identify potential anti-diabetic peptides with α-glucosidase and α-amylase inhibitory activities. After the Alcalase hydrolysis, egg white protein hydrolysates were purified and identified by LC–MS–MS. Eight identified peptides were further synthesized by the Fmoc solid-phase synthesis. The anti-diabetic activities of these synthetic peptides were assessed using enzymatic inhibitory assays against the α-glucosidase and α-amylase. Among the eight peptides, peptide RVPSLM was discovered as a potential α-glucosidase inhibitor with an IC50value at 23.07μmolL−1. However, it did not exhibit a visible or detectable inhibitory efficiency on the α-amylase. These studies indicate the potential of using egg white protein hydrolysates as a functional food product with the anti-diabetic activity.
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