Evaluating a targeted multiple reaction monitoring approach to global untargeted lipidomic analyses of human plasma.
Evaluating a targeted multiple reaction monitoring approach to global untargeted lipidomic analyses of human plasma.
复制标题
DOI：
10.1002/rcm.8911
复制
发表时间：
2020-11-30
影响因子：
2
通讯作者：
Robinson, Rena A. S.
中科院分区：
文献类型：
--
作者：
Khan, Mostafa J.;Codreanu, Simona G.;Goyal, Sandeep;Wages, Phillip A.;Gorti, Santosh K. K.;Pearson, Mackenzie J.;Uribe, Isabel;Sherrod, Stacy D.;McLean, John A.;Porter, Ned A.;Robinson, Rena A. S.
Lipidyzer平台最近更新了SCIEX QTRAP 6500+质谱仪，提供包括1150种不同脂质的靶向脂质组学分析。我们使用人类血浆样本评估了这种靶向方法，并使用高分辨率Q Exactive HF Orbitrap质谱仪将结果与全球非靶向脂质组学方法进行了比较。用改良的Bligh-Dyer法提取人血浆样本（N = 5）中的脂质。使用Thermo Orbitrap Q Exactive HF质谱仪进行全局非靶向分析，然后使用Progenesis QI软件进行数据分析。采用QTRAP 6500+质谱仪进行基于多反应监测（MRM）的靶向分析，然后使用SCIEX OS软件进行数据分析。样品分别在三天内注射，以评估两种方法的可重复性。总的来说，两种方法从11种脂类中鉴定出465种脂类，其中159种在方法之间相似，168种脂类是MRM方法所特有的，138种脂类是非靶向方法所特有的。磷脂酰胆碱和磷脂酰乙醇胺是最常用的非靶向方法，而三酰甘油是最常用的靶向MRM方法。靶向MRM方法在三天内比非靶向方法具有更一致的相对丰度。总体而言，所有脂质类别日间比较的变异系数在非靶向方法中为~ 23%，在靶向MRM方法中为~ 9%。靶向MRM方法识别的脂类数量与传统的非靶向方法相似，但更好地代表了两种方法共同识别的11种脂类。基于所采用的分离方法，传统的非靶向方法可以更好地检测磷脂酰胆碱和鞘磷脂脂类。当使用一小组血浆样本进行测试时，靶向MRM方法比非靶向方法具有更低的日间变异性。这些研究强调了使用靶向MRM方法进行人血浆脂质组学分析的优势。
The Lipidyzer platform was recently updated on a SCIEX QTRAP 6500+ mass spectrometer and offers a targeted lipidomics assay including 1150 different lipids. We evaluated this targeted approach using human plasma samples and compared the results against a global untargeted lipidomics method using a high-resolution Q Exactive HF Orbitrap mass spectrometer. Lipids from human plasma samples (N = 5) were extracted using a modified Bligh–Dyer approach. A global untargeted analysis was performed using a Thermo Orbitrap Q Exactive HF mass spectrometer, followed by data analysis using Progenesis QI software. Multiple reaction monitoring (MRM)-based targeted analysis was performed using a QTRAP 6500+ mass spectrometer, followed by data analysis using SCIEX OS software. The samples were injected on three separate days to assess reproducibility for both approaches. Overall, 465 lipids were identified from 11 lipid classes in both approaches, of which 159 were similar between the methods, 168 lipids were unique to the MRM approach, and 138 lipids were unique to the untargeted approach. Phosphatidylcholine and phosphatidylethanolamine species were the most commonly identified using the untargeted approach, while triacylglycerol species were the most commonly identified using the targeted MRM approach. The targeted MRM approach had more consistent relative abundances across the three days than the untargeted approach. Overall, the coefficient of variation for inter-day comparisons across all lipid classes was ∼ 23% for the untargeted approach and ∼ 9% for the targeted MRM approach. The targeted MRM approach identified similar numbers of lipids to a conventional untargeted approach, but had better representation of 11 lipid classes commonly identified by both approaches. Based on the separation methods employed, the conventional untargeted approach could better detect phosphatidylcholine and sphingomyelin lipid classes. The targeted MRM approach had lower inter-day variability than the untargeted approach when tested using a small group of plasma samples. These studies highlight the advantages in using targeted MRM approaches for human plasma lipidomics analysis.
登录
查看更多内容
影响因子：
3.7
作者：
Ishikawa S;Tateya I;Hayasaka T;Masaki N;Takizawa Y;Ohno S;Kojima T;Kitani Y;Kitamura M;Hirano S;Setou M;Ito J
通讯作者：
Ito J
影响因子：
7.4
作者：
Fhaner, Cassie J.;Liu, Sichang;Ji, Hong;Simpson, Richard J.;Reid, Gavin E.
通讯作者：
Reid, Gavin E.
影响因子：
2.1
作者：
Han, Xianlin
通讯作者：
Han, Xianlin
影响因子：
2.9
作者：
Han, Xianlin;Yang, Jingyue;Gross, Richard W.
通讯作者：
Gross, Richard W.
影响因子：
6.5
作者：
Ollero, Mario;Astarita, Giuseppe;Edelman, Aleksander
通讯作者：
Edelman, Aleksander