Glycyrrhizin as antiviral agent against Hepatitis C Virus.
Glycyrrhizin as antiviral agent against Hepatitis C Virus.
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DOI：
10.1186/1479-5876-9-112
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发表时间：
2011-07-18
影响因子：
7.4
通讯作者：
Riazuddin S
中科院分区：
文献类型：
--
作者：
Ashfaq UA;Masoud MS;Nawaz Z;Riazuddin S
丙型肝炎病毒是慢性肝病的主要病因，可导致永久性肝损伤、肝细胞癌和死亡。目前可用的干扰素加利巴韦林治疗，由于不良副作用，如贫血、抑郁、疲劳和“流感样”症状，疗效有限。几个世纪以来，草药一直被用来治疗包括病毒性疾病在内的不同疾病，并已成为治疗细菌和病毒性疾病的新化合物的主要来源。本研究旨在研究甘草酸（Glycyrrhizin， GL）对丙型肝炎病毒的抗病毒作用。为此，用无毒剂量的GL处理HCV感染的肝细胞，并通过定量实时RT-PCR检测HCV滴度。我们的研究结果表明，GL对HCV滴度的抑制呈剂量依赖性，浓度为14±2 μg时，HCV滴度降低50%。与干扰素α进行了比较研究，以研究抗病毒化合物与干扰素α 2a之间的协同作用，如果有的话。我们的数据显示，GL与干扰素联合使用时表现出协同效应。此外，用HCV 3a核心质粒瞬时转染肝细胞验证了这些结果。结果证明，在GAPDH保持不变的情况下，GL在mRNA和蛋白水平上均具有剂量依赖性抑制HCV 3a核心基因的表达。我们的研究结果表明，GL抑制HCV全长病毒颗粒和HCV核心基因的表达或功能呈剂量依赖性，并与干扰素具有协同作用。未来，GL联合干扰素将是治疗HCV感染的更好选择。
Hepatitis C virus is a major cause of chronic liver diseases which can lead to permanent liver damage, hepatocellular carcinoma and death. The presently available treatment with interferon plus ribavirin, has limited benefits due to adverse side effects such as anemia, depression, fatigue, and "flu-like" symptoms. Herbal plants have been used for centuries against different diseases including viral diseases and have become a major source of new compounds to treat bacterial and viral diseases. The present study was design to study the antiviral effect of Glycyrrhizin (GL) against HCV. For this purpose, HCV infected liver cells were treated with GL at non toxic doses and HCV titer was measured by Quantitative real time RT-PCR. Our results demonstrated that GL inhibit HCV titer in a dose dependent manner and resulted in 50% reduction of HCV at a concentration of 14 ± 2 μg. Comparative studies were made with interferon alpha to investigate synergistic effects, if any, between antiviral compound and interferon alpha 2a. Our data showed that GL exhibited synergistic effect when combined with interferon. Moreover, these results were verified by transiently transfecting the liver cells with HCV 3a core plasmid. The results proved that GL dose dependently inhibit the expression of HCV 3a core gene both at mRNA and protein levels while the GAPDH remained constant. Our results suggest that GL inhibit HCV full length viral particles and HCV core gene expression or function in a dose dependent manner and had synergistic effect with interferon. In future, GL along with interferon will be better option to treat HCV infection.
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影响因子：
4.2
作者：
Iino, S;Tango, T;Suzuki, H
通讯作者：
Suzuki, H
影响因子：
29.4
作者：
Polyak, Stephen J.;Morishima, Chihiro;Lee, David Y. -W.
通讯作者：
Lee, David Y. -W.
影响因子：
7.6
作者：
Sato, H;Goto, W;Shiraki, K
通讯作者：
Shiraki, K
影响因子：
7.6
作者：
CRANCE, JM;LEVEQUE, F;DELOINCE, R
通讯作者：
DELOINCE, R
DOI：
10.1007/bf02171049
发表时间：
1992-10-01
期刊：
BIOTHERAPY
影响因子：
--
作者：
KIMURA, M;WATANABE, H;ABO, T
通讯作者：
ABO, T