A multicopper oxidase-related protein is essential for insect viability, longevity and ovary development.
A multicopper oxidase-related protein is essential for insect viability, longevity and ovary development.
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DOI：
10.1371/journal.pone.0111344
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Gorman MJ
中科院分区：
文献类型：
--
作者：
Peng Z;Green PG;Arakane Y;Kanost MR;Gorman MJ
典型的多铜氧化酶（MCOs）有十个保守的组氨酸和一个保守的半胱氨酸配位四个铜原子。这些铜离子是氧化酶活性所必需的。在对昆虫多铜氧化酶相关蛋白的研究中，我们发现了一个被命名为多铜氧化酶相关蛋白（MCORP）的基因。MCORPs与mco序列相似，但缺乏铜配位残基。我们在许多昆虫物种中发现了MCORP同源物，但在其他无脊椎动物或脊椎动物中没有发现。我们预测由于缺乏铜配位残基，MCORPs将缺乏氧化酶活性。为了验证这一预测，我们纯化了重组Tribolium castaneum（红粉甲虫）MCORP，并在不同底物下分析了其酶活性。不出所料，未检测到氧化酶活性。为了研究MCORP在体内的功能，我们分析了TcMCORP和冈比亚按蚊（Anopheles gambiae） MCORP的表达谱，并评估了rnai介导的敲低表型。我们发现这两种MCORPs在我们分析的所有组织中都以低水平组成性表达。将TcMCORP dsRNA注射到幼虫体内，在成虫羽化前死亡率为100%，死亡主要发生在舌蛹期或舌成虫后期。将TcMCORP的dsRNA注射到pharate蛹中，大约20%的处理过的昆虫在蛹向成虫转变的过程中死亡，其余昆虫的寿命大大缩短。此外，在雌性中，TcMCORP的敲低阻止了卵母细胞成熟，从而大大减少了产卵数量。这些结果表明，TcMCORP是一个重要的基因，其功能是生殖所必需的。了解MCORP在昆虫生理中的作用有助于开发新的害虫防治策略。
Typical multicopper oxidases (MCOs) have ten conserved histidines and one conserved cysteine that coordinate four copper atoms. These copper ions are required for oxidase activity. During our studies of insect MCOs, we discovered a gene that we named multicopper oxidase-related protein (MCORP). MCORPs share sequence similarity with MCOs, but lack many of the copper-coordinating residues. We identified MCORP orthologs in many insect species, but not in other invertebrates or vertebrates. We predicted that MCORPs would lack oxidase activity due to the absence of copper-coordinating residues. To test this prediction, we purified recombinant Tribolium castaneum (red flour beetle) MCORP and analyzed its enzymatic activity using a variety of substrates. As expected, no oxidase activity was detected. To study MCORP function in vivo, we analyzed expression profiles of TcMCORP and Anopheles gambiae (African malaria mosquito) MCORP, and assessed RNAi-mediated knockdown phenotypes. We found that both MCORPs are constitutively expressed at a low level in all of the tissues we analyzed. Injection of TcMCORP dsRNA into larvae resulted in 100% mortality prior to adult eclosion, with death occurring mainly during the pharate pupal stage or late pharate adult stage. Injection of TcMCORP dsRNA into pharate pupae resulted in the death of approximately 20% of the treated insects during the pupal to adult transition and a greatly shortened life span for the remaining insects. In addition, knockdown of TcMCORP in females prevented oocyte maturation and, thus, greatly decreased the number of eggs laid. These results indicate that TcMCORP is an essential gene and that its function is required for reproduction. An understanding of the role MCORP plays in insect physiology may help to develop new strategies for controlling insect pests.
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冈比亚按蚊中多型氧化酶基因家族的表征。
影响因子：
3.8
作者：
Gorman, Maureen J.;Dittmer, Neal T.;Marshall, Jeremy L.;Kanost, Michael R.
通讯作者：
Kanost, Michael R.
影响因子：
4.8
作者：
Palmieri, G;Giardina, P;Sannia, G
通讯作者：
Sannia, G
DOI：
10.1073/pnas.0504982102
发表时间：
2005-08-09
影响因子：
11.1
作者：
Arakane, Y;Muthukrishnan, S;Kramer, KJ
通讯作者：
Kramer, KJ
影响因子：
3.8
作者：
Dittmer, NT;Suderman, RJ;Kanost, MR
通讯作者：
Kanost, MR
取代酚的氧化还原电势，漆酶氧化和抗高清活性。
影响因子：
3.5
作者：
Prasain, Keshar;Nguyen, Thi D. T.;Gorman, Maureen J.;Barrigan, Lydia M.;Peng, Zeyu;Kanost, Michael R.;Syed, Lateef U.;Li, Jun;Zhu, Kun Yan;Hua, Duy H.
通讯作者：
Hua, Duy H.