Physisorbed surface coatings for poly(dimethylsiloxane) and quartz microfluidic devices.
Physisorbed surface coatings for poly(dimethylsiloxane) and quartz microfluidic devices.
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聚（二甲基硅氧烷）和石英微流体装置的物理表面涂层。
DOI：
10.1007/s00216-011-5301-z
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发表时间：
2011-10
影响因子：
4.3
通讯作者：
Ros, A.
中科院分区：
文献类型：
--
作者：
Viefhues, M.;Manchanda, S.;Chao, T. -C.;Anselmetti, D.;Regtmeier, J.;Ros, A.
微流控装置的表面修饰对于成功的生物分析应用至关重要。在这里，我们研究了三种不同的涂层用于石英和聚二甲基硅氧烷(PDMS)表面。我们采用了一种商标为F108的三嵌段共聚物，即聚(L-赖氨酸)-g-聚乙二醇(PLL-聚乙二醇酯)，以及n-十二烷基-β-d-麦芽糖苷和甲基纤维素杂化涂层(DDM/MC)。通过测量电渗流(EOF)、接触角和防止蛋白质吸附来表征这些涂层的影响。此外，我们还研究了静态涂层和动态涂层的影响，静态涂层是指在测量前与涂层试剂的孵化，而动态涂层是在测量过程中涂层试剂存在的情况。我们发现，在PDMS和石英表面的所有涂层都降低了EOF，增加了EOF的重现性，减少了对蛋白质的吸附，并改善了表面的润湿性。在所测试的涂层策略中，DDM/MC和F108的动态涂层显示出最大程度的降低EOF和蛋白质吸附，同时具有最好的EOF长期稳定性。对于PLL-PEG，观察到EOF方向的反转。有趣的是，使用F108的静态表面涂层策略被证明与使用这种嵌段共聚物的动态涂层一样有效地防止蛋白质吸附。这些发现将为PDMS和石英微流控器件的涂层策略提供优化的参数选择，其中EOF的控制和减少生物污垢是必不可少的。
Surface modifications of microfluidic devices are of essential importance for successful bioanalytical applications. Here, we investigate three different coatings for quartz and poly(dimethylsiloxane) (PDMS) surfaces. We employed a triblock copolymer with trade name F108, poly (l-lysine)-g-poly(ethylene glycol) (PLL-PEG), as well as the hybrid coating n-dodecyl-β-d-maltoside and methyl cellulose (DDM/MC). The impact of these coatings was characterized by measuring the electroosmotic flow (EOF), contact angle, and prevention of protein adsorption. Furthermore, we investigated the influence of static coatings, i.e., the incubation with the coating agent prior to measurements, and dynamic coatings, where the coating agent was present during the measurement. We found that all coatings on PDMS as well as quartz reduced EOF, increased reproducibility of EOF, reduced protein adsorption, and improved the wettability of the surfaces. Among the coating strategies tested, the dynamic coatings with DDM/MC and F108 demonstrated maximal reduction of EOF and protein adsorption and simultaneously best long-term stability concerning EOF. For PLL-PEG, a reversal in the EOF direction was observed. Interestingly, the static surface coating strategy with F108 proved to be as effective to prevent protein adsorption as dynamic coating with this block copolymer. These findings will allow optimized parameter choices for coating strategies on PDMS and quartz microfluidic devices in which control of EOF and reduced biofouling are indispensable.
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影响因子：
3.6
作者：
De Smet, Nele;Rymarczyk-Machal, Monika;Schacht, Etienne
通讯作者：
Schacht, Etienne
影响因子：
7.4
作者：
Lucy, CA;Underhill, RS
通讯作者：
Underhill, RS
影响因子：
7.4
作者：
Lai, SY;Cao, X;Lee, LJ
通讯作者：
Lee, LJ
影响因子：
3.9
作者：
Huang, NP;Michel, R;Spencer, ND
通讯作者：
Spencer, ND
影响因子：
3.9
作者：
Hellmich, W;Regtmeier, J;Ros, A
通讯作者：
Ros, A