The absence of Arabidopsis-type telomeres in Cestrum and closely related genera Vestia and Sessea (Solanaceae): first evidence from eudicots.
The absence of Arabidopsis-type telomeres in Cestrum and closely related genera Vestia and Sessea (Solanaceae): first evidence from eudicots.
复制标题
Cestrum 和密切相关的 Vestia 属和 Sessea 属（茄科）中缺乏拟南芥型端粒：来自真双子叶植物的第一个证据。
DOI：
--
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
J. Fajkus
中科院分区：
文献类型：
--
作者：
E. Sýkorová;K. Y. Lim;M. Chase;S. Knapp;I. Leitch;A. Leitch;J. Fajkus
使用狭缝印迹和荧光原位杂交（FISH），我们没有发现任何证据的存在下，在我们调查的任何蝙蝠属物种的染色体末端的costrossis型端粒序列（TTTAGGG）n。探测人类型端粒（TTAGGG）n也没有发现信号。然而，聚合酶链反应实验表明，有短长度的序列TTTAGGG分散在基因组中，但这些序列几乎肯定太短，作为功能端粒，即使他们在染色体末端。对相关属Vestia和Sessea的分析表明，它们也缺乏Escherichiopsis型端粒，并且这些序列在这些属的共同祖先中丢失。我们发现，所调查的蝙蝠属物种有特别大的平均染色体大小。我们讨论这是否是一个替代端粒末端维护系统的后果。
Using slot-blot and fluorescent in situ hybridization (FISH), we found no evidence for the presence of the Arabidopsis-type telomeric sequence (TTTAGGG)n at the chromosome termini in any of the Cestrum species we investigated. Probing for the human-type telomere (TTAGGG)n also revealed no signal. However, polymerase chain reaction experiments indicated that there are short lengths of the sequence TTTAGGG dispersed in the genome but that these sequences are almost certainly too short to act as functional telomeres even if they were at the chromosome termini. An analysis of related genera Vestia and Sessea indicates that they too lack the Arabidopsis-type telomere, and the sequences were lost in the common ancestor of these genera. We found that the Cestrum species investigated had particularly large mean chromosome sizes. We discuss whether this is a consequence of alternative telomere end maintenance systems.
影响因子：
56.9
作者：
Nakamura, TM;Cooper, JP;Cech, TR
通讯作者：
Cech, TR
DOI：
10.1073/pnas.85.18.6622
发表时间：
1988-09-01
影响因子：
11.1
作者：
MOYZIS, RK;BUCKINGHAM, JM;WU, JR
通讯作者：
WU, JR