Three-dimensional fabrication of cell-laden biodegradable poly(ethylene glycol-co-depsipeptide) hydrogels by visible light stereolithography.
Three-dimensional fabrication of cell-laden biodegradable poly(ethylene glycol-co-depsipeptide) hydrogels by visible light stereolithography.
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DOI：
10.1039/c5tb01468a
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发表时间：
2015-11-14
期刊：
影响因子：
--
通讯作者：
Yang Y
中科院分区：
文献类型：
--
作者：
Elomaa L;Pan CC;Shanjani Y;Malkovskiy A;Seppälä JV;Yang Y
立体光刻技术(SLA)在制备用于组织工程和制药的具有仿生复杂性的细胞水凝胶方面具有很大的前景。然而，用于SLA的可生物降解的光交联型水凝胶聚合物的可用性非常有限。在这项研究中，合成了一种可生物降解的光交联型SLA大分子单体，合成了一种可溶于水的甲基丙烯酸酯共聚乙二醇共缩肽。结构分析证实了聚合物主链中含有可生物降解的肽基、酯基和光交联甲基丙烯酸酯基团。将新的大分子单体与RGDS多肽结合，在无细胞和有细胞存在的情况下制备SLA水凝胶。随着SLA光照时间的延长，水凝胶的机械硬度从3±1kPa增加到38±13kPa.样品在PBS中的总质量损失在7d内为13%~21%，在24d内为35%~66%。由于降解，在PBS中孵育7天内，机械硬度降低了一个数量级。在体外细胞培养研究中，包裹的内皮细胞在水凝胶中增殖。该大分子聚合物被进一步应用于SLA中，以制备分叉管状结构作为初步的血管移植物。这种新的可生物降解、可光交联的聚合物是对目前基于SLA制造细胞负载组织工程结构的非常有限的材料选择的重大补充。利用立体光刻技术和一种新型的可生物降解、可光交联的聚乙二醇共聚多肽预聚体，制备了三维可定义的细胞载水凝胶结构。
Stereolithography (SLA) holds great promise in fabrication of cell-laden hydrogels with biomimetic complexity for use in tissue engineering and pharmaceutics. However, the availability of biodegradable photocrosslinkable hydrogel polymers for SLA is very limited. In this study, a water-soluble methacrylated poly(ethylene glycol-co-depsipeptide) was synthesized to yield a biodegradable photocrosslinkable macromer for SLA. Structural analysis confirmed the inclusion of biodegradable peptide and ester groups and photocrosslinkable methacrylate groups into the polymer backbone. The new macromer combined with RGDS peptide was used for SLA fabrication of hydrogels in absence and presence of cells. With the increasing light exposure time in SLA, mechanical stiffness of the hydrogels increased from 3 ± 1 kPa to 38 ± 13 kPa. Total mass loss of the samples within 7 days in PBS was 13%–21% and within 24 days 35%–66%. Due to degradation, the mechanical stiffness decreased by one order magnitude within 7-day incubation in PBS. Encapsulated endothelial cells proliferated in the hydrogels during 10-day in vitro cell culturing study. The macromer was further used in SLA to fabricate bifurcating tubular structures as preliminary vessel grafts. The new biodegradable, photocrosslinkable polymer is a significant addition to the very limited material selection currently available for SLA-based fabrication of cell-laden tissue engineering constructs. 3D defined cell-laden hydrogel constructs were fabricated using stereolithography and a new biodegradable photocrosslinkable poly(ethylene glycol-co-depsipeptide) prepolymer.
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影响因子：
10.8
作者：
Seck, Tetsu M.;Melchels, Ferry P. W.;Grijpma, Dirk W.
通讯作者：
Grijpma, Dirk W.
影响因子：
3.4
作者：
Dikovsky, Daniel;Bianco-Peled, Havazelet;Seliktar, Dror
通讯作者：
Seliktar, Dror
影响因子：
9.1
作者：
Elomaa, Laura;Kokkari, Anne;Seppala, Jukka V.
通讯作者：
Seppala, Jukka V.
影响因子：
--
作者：
Elomaa, Laura;Kang, Yunqing;Yang, Yunzhi
通讯作者：
Yang, Yunzhi
影响因子：
5.5
作者：
Lin, HQ;Kai, T;Kalika, DS
通讯作者：
Kalika, DS