Downstream Neighbor of Son Overexpression is Associated With Breast Cancer Progression and a Poor Prognosis.
Downstream Neighbor of Son Overexpression is Associated With Breast Cancer Progression and a Poor Prognosis.
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Son 下游邻居过度表达与乳腺癌进展和不良预后相关
DOI：
10.4048/jbc.2022.25.e26
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发表时间：
2022-08
影响因子：
2.4
通讯作者：
Dai, Xuanxuan
中科院分区：
文献类型：
--
作者：
Qi, Yufeng;Wu, Haodong;Liu, Conghui;Zheng, Danni;Yan, Congzhi;Hu, Wenjing;Zhang, Xiaohua;Dai, Xuanxuan
乳腺癌（BC）的发病率逐年上升。下游邻近子（DONSON）严重影响细胞周期进程，并保持稳定的基因组特性;然而，其对BC生长和进展的相关影响需要深入研究。DONSON上调在公共数据库中得到验证。使用定量逆转录聚合酶链反应测定匹配的BC和邻近组织和细胞系（MDA-MB-231、BT-549和HS-578 T）中的DONSON表达。通过体外细胞凋亡、侵袭、迁移和增殖试验，确定DONSON在BC细胞系中的功能。然后，使用蛋白质印迹分析，确定DONSON下游蛋白质的水平。与对照相比，DONSON在BC组织和细胞系中以更高的水平表达。DONSON基因敲低可促进BC细胞凋亡，并限制BC细胞的增殖、迁移、侵袭和S/G2转换。此外，DONSON过表达在体外促进BC细胞增殖并抑制凋亡。此外，DONSON敲低降低了细胞周期蛋白A1和细胞周期蛋白依赖性激酶2的水平。此外，DONSON敲低限制了上皮-间充质转化的进展。DONSON严重影响BC生长，并作为后续治疗疗效的可能靶点和标志物。
The incidence rate of breast cancer (BC) has increased annually. Downstream neighbor of son (DONSON) critically affects cell cycle progression and maintains stable genomic properties; however, its relevant effects on BC growth and progression require in-depth investigation. DONSON upregulation was validated in public databases. DONSON expression in matched BC and adjacent tissues and cell lines (MDA-MB-231, BT-549, and HS-578T) was determined using quantitative reverse transcription polymerase chain reaction. In vitro apoptosis, invasion, migration, and proliferation tests were performed to ascertain the functions of DONSON in BC cell lines. Then, using western blot analysis, the levels of DONSON downstream proteins were determined. Compared to the control, DONSON was expressed at higher levels in BC tissues and cell lines. DONSON knockdown facilitated apoptosis and limited proliferation, migration, invasion, and S/G2 transition of BC cells in vitro. Furthermore, DONSON overexpression promoted BC cell proliferation and inhibited apoptosis in vitro. Moreover, DONSON knockdown reduced cyclin A1 and cyclin-dependent kinase 2 levels. Moreover, DONSON knockdown limited the progression of epithelial-mesenchymal transition. DONSON critically affects BC growth and serves as a possible target and marker for the efficacy of subsequent therapies.
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