PhosphoSitePlus: a comprehensive resource for investigating the structure and function of experimentally determined post-translational modifications in man and mouse.
PhosphoSitePlus: a comprehensive resource for investigating the structure and function of experimentally determined post-translational modifications in man and mouse.
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DOI：
10.1093/nar/gkr1122
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发表时间：
2012-01
影响因子：
14.9
通讯作者：
Sullivan M
中科院分区：
文献类型：
--
作者：
Hornbeck PV;Kornhauser JM;Tkachev S;Zhang B;Skrzypek E;Murray B;Latham V;Sullivan M
PhosphoSitePlus（http：www.phosphosite.org）是一个开放的，全面的，手动策划和互动的资源，用于研究实验观察到的翻译后修饰，主要是人类和小鼠蛋白质。它包含130 000个非冗余修饰位点，主要是磷酸化、泛素化和乙酰化。该界面的设计是为了清晰和易于导航。从主页上，用户可以启动简单或复杂的搜索，并按疾病、组织或细胞系浏览高通量数据集。可通过特定治疗、蛋白质类型、结构域、细胞组分、疾病、细胞类型、细胞系、组织和序列或基序来限制生物活性。用户只需点击几下鼠标，就可以进入含有已知被修饰的侧链的位点、序列、结构域图和分子可视化的底物页面或蛋白质页面;进入含有关于修饰位点如何与特定蛋白质和细胞过程的功能相关的信息的网站页面，并进入汇总一条记录的详细信息的精选信息页面。可以下载PyMOL和Chimera脚本，这些脚本可以对修饰的残基上的反应基团进行着色。旨在促进蛋白质组学分析的功能包括下载修饰位点、激酶-底物数据集、序列标志生成器、Cytoscape插件和BioPAX下载，以实现PhosphoSitePlus®中激酶-底物相互作用的途径可视化。
PhosphoSitePlus (http://www.phosphosite.org) is an open, comprehensive, manually curated and interactive resource for studying experimentally observed post-translational modifications, primarily of human and mouse proteins. It encompasses 1 30 000 non-redundant modification sites, primarily phosphorylation, ubiquitinylation and acetylation. The interface is designed for clarity and ease of navigation. From the home page, users can launch simple or complex searches and browse high-throughput data sets by disease, tissue or cell line. Searches can be restricted by specific treatments, protein types, domains, cellular components, disease, cell types, cell lines, tissue and sequences or motifs. A few clicks of the mouse will take users to substrate pages or protein pages with sites, sequences, domain diagrams and molecular visualization of side-chains known to be modified; to site pages with information about how the modified site relates to the functions of specific proteins and cellular processes and to curated information pages summarizing the details from one record. PyMOL and Chimera scripts that colorize reactive groups on residues that are modified can be downloaded. Features designed to facilitate proteomic analyses include downloads of modification sites, kinase–substrate data sets, sequence logo generators, a Cytoscape plugin and BioPAX download to enable pathway visualization of the kinase–substrate interactions in PhosphoSitePlus®.
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影响因子：
14.9
作者：
Keshava Prasad TS;Goel R;Kandasamy K;Keerthikumar S;Kumar S;Mathivanan S;Telikicherla D;Raju R;Shafreen B;Venugopal A;Balakrishnan L;Marimuthu A;Banerjee S;Somanathan DS;Sebastian A;Rani S;Ray S;Harrys Kishore CJ;Kanth S;Ahmed M;Kashyap MK;Mohmood R;Ramachandra YL;Krishna V;Rahiman BA;Mohan S;Ranganathan P;Ramabadran S;Chaerkady R;Pandey A
通讯作者：
Pandey A
影响因子：
7.4
作者：
MacCoss, MJ;Wu, CC;Yates, JR
通讯作者：
Yates, JR
影响因子：
14.9
作者：
Dinkel H;Chica C;Via A;Gould CM;Jensen LJ;Gibson TJ;Diella F
通讯作者：
Diella F
影响因子：
3.7
作者：
Gu TL;Deng X;Huang F;Tucker M;Crosby K;Rimkunas V;Wang Y;Deng G;Zhu L;Tan Z;Hu Y;Wu C;Nardone J;MacNeill J;Ren J;Reeves C;Innocenti G;Norris B;Yuan J;Yu J;Haack H;Shen B;Peng C;Li H;Zhou X;Liu X;Rush J;Comb MJ
通讯作者：
Comb MJ
影响因子：
14.9
作者：
Finn RD;Mistry J;Tate J;Coggill P;Heger A;Pollington JE;Gavin OL;Gunasekaran P;Ceric G;Forslund K;Holm L;Sonnhammer EL;Eddy SR;Bateman A
通讯作者：
Bateman A