Identification and Characterization of Circular RNAs in Mammary Tissue from Holstein Cows at Early Lactation and Non-Lactation.
Identification and Characterization of Circular RNAs in Mammary Tissue from Holstein Cows at Early Lactation and Non-Lactation.
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DOI：
10.3390/biom12030478
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发表时间：
2022-03-21
期刊：
影响因子：
5.5
通讯作者：
Mao Y
中科院分区：
文献类型：
--
作者：
Liang Y;Gao Q;Wang H;Guo M;Arbab AAI;Nazar M;Li M;Yang Z;Karrow NA;Mao Y
本研究对泌乳早期和非泌乳期荷斯坦奶牛乳腺组织中的环状RNA（circRNA）进行了鉴定和比较。经过分析，鉴定出10，684个circRNA，范围从48到99，406 bp，平均大小为882 bp。circRNA主要分布在1 ~ 11号染色体上，89.89%的circRNA属于正义重叠型。含有circRNA的外显子的范围从1到47，并集中从1到5。与非泌乳奶牛相比，87个circRNA在泌乳高峰期奶牛中差异显著。有68个上调的circRNA和19个下调的circRNA。circRNA的富集分析显示，GO分析主要集中在免疫应答、甘油三酯转运、T细胞受体信号通路等方面，通路分析主要集中在精氨酸-细胞因子受体相互作用、T辅助细胞17细胞分化、脂肪酸生物合成、JAK-STAT信号通路、为circRNA连接位点的两个近端设计特异性引物，以允许对四个随机选择的circRNA进行PCR验证，并进行circRNA-miRNA相互作用研究。本研究揭示了奶牛泌乳早期和非泌乳期乳腺组织中circRNA的表达谱和特征，为circRNA的功能和机制研究提供了丰富的信息，也为奶牛泌乳研究提供了潜在的候选miRNA基因。
In this study, circular RNAs (circRNAs) from Holstein cow mammary tissues were identified and compared between early lactation and non-lactation. After analysis, 10,684 circRNAs were identified, ranging from 48 to 99,406 bp, and the average size was 882 bp. The circRNAs were mainly distributed on chromosomes 1 to 11, and 89.89% of the circRNAs belonged to sense-overlapping circRNA. The exons contained with circRNAs ranged from 1 to 47 and were concentrated from 1 to 5. Compared with the non-lactating cows, 87 circRNAs were significantly differentially expressed in the peak lactation cows. There were 68 upregulated circRNAs and 19 downregulated circRNAs. Enrichment analysis of circRNAs showed that GO analysis mainly focused on immune response, triglyceride transport, T cell receptor signaling pathway, etc. Pathway analysis mainly focused on cytokine-cytokine receptor interaction, T helper 17 cell differentiation, fatty acid biosynthesis, the JAK-STAT signaling pathway, etc. Specific primers were designed for two proximal ends of the circRNA junction sites to allow for PCR validation of four randomly selected circRNAs and carry out circRNA-miRNA interaction research. This study revealed the expression profile and characteristics of circRNAs in mammary tissue from Holstein cows at early lactation and non-lactation, thus providing rich information for the study of circRNA functions and mechanisms, as well as potential candidate miRNA genes for studying lactation in Holstein cows.
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影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
3.7
作者：
Das S;Ghosal S;Sen R;Chakrabarti J
通讯作者：
Chakrabarti J
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DOI：
10.1093/dnares/dsx022
发表时间：
2017-10-01
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Liang G;Yang Y;Niu G;Tang Z;Li K
通讯作者：
Li K
circRNA生物合成的调控
影响因子：
4.1
作者：
Chen, Ling-Ling;Yang, Li
通讯作者：
Yang, Li
DOI：
10.1016/j.omtn.2020.08.016
发表时间：
2020-12-04
期刊：
MOLECULAR THERAPY NUCLEIC ACIDS
影响因子：
--
作者：
Freage, Lina;Jamal, Deana;Mallikaratchy, Prabodhika R.
通讯作者：
Mallikaratchy, Prabodhika R.