Roles of RbcX in Carboxysome Biosynthesis in the Cyanobacterium Synechococcus elongatus PCC7942.
Roles of RbcX in Carboxysome Biosynthesis in the Cyanobacterium Synechococcus elongatus PCC7942.
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DOI：
10.1104/pp.18.01217
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发表时间：
2019-01
期刊：
影响因子：
7.4
通讯作者：
Liu LN
中科院分区：
文献类型：
--
作者：
Huang F;Vasieva O;Sun Y;Faulkner M;Dykes GF;Zhao Z;Liu LN
RbcX是Rubisco生物发生的伴侣，参与长聚球藻7942的羧体组装。Rubisco是光合作用过程中调节大气CO2固定的重要酶。在蓝藻中，Rubisco酶被密集地包装和包裹在一个被称为羧体的专门细胞器中。明确的Rubisco组装和羧体形成是有效固定CO2的关键。许多伴侣蛋白，包括RbcX，对于正确的蛋白质折叠和Rubisco组装是必不可少的。在这项研究中，我们用分子、生化和活细胞荧光成像的方法研究了RbcX在蓝藻细长聚球藻PCC7942(Syn7942)中的体内功能。我们的结果表明，rbcX基因的遗传缺失影响了Rubisco的丰度，以及羧体的形成和空间分布。此外，RbcX作为羧体的一种成分，与Rubisco酶表现出动态的相互作用。这些体内观察提供了来自Syn7942的RbcX在介导羧体组装中的作用的洞察力。了解Rubisco组装和羧体生物发生的分子机制将为在异质生物，特别是植物中设计功能性二氧化碳固定复合体，以提高光合作用和农业生产力提供必要的信息。
RbcX, the chaperone for Rubisco biogenesis, is involved in carboxysome assembly in Synechococcus elongatus 7942. Rubisco is the essential enzyme mediating the fixation of atmospheric CO2 during photosynthesis. In cyanobacteria, Rubisco enzymes are densely packed and encapsulated in a specialized organelle known as the carboxysome. Well-defined Rubisco assembly and carboxysome formation are pivotal for efficient CO2 fixation. Numerous chaperone proteins, including RbcX, are essential for proper protein folding and Rubisco assembly. In this study, we investigated the in vivo function of RbcX in the cyanobacterium Synechococcus elongatus PCC 7942 (Syn7942) using molecular, biochemical, and live-cell fluorescence imaging approaches. Our results show that genetic deletion of the rbcX gene affects Rubisco abundance, as well as carboxysome formation and spatial distribution. Moreover, RbcX appears as one component of the carboxysome and shows a dynamic interaction with Rubisco enzymes. These in vivo observations provide insight into the role of RbcX from Syn7942 in mediating carboxysome assembly. Understanding the molecular mechanism underlying Rubisco assembly and carboxysome biogenesis will provide essential information required for engineering functional CO2-fixing complexes in heterogeneous organisms, especially plants, with the aim of boosting photosynthesis and agricultural productivity.
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