Evidence of a critical role for cellodextrin transporte 2 (CDT-2) in both cellulose and hemicellulose degradation and utilization in Neurospora crassa.
Evidence of a critical role for cellodextrin transporte 2 (CDT-2) in both cellulose and hemicellulose degradation and utilization in Neurospora crassa.
复制标题
纤维糊精转运蛋白 2 (CDT-2) 在粗糙脉孢菌的纤维素和半纤维素降解和利用中发挥关键作用的证据
DOI：
10.1371/journal.pone.0089330
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Ma Y
中科院分区：
文献类型：
--
作者：
Cai P;Gu R;Wang B;Li J;Wan L;Tian C;Ma Y
CDT-1和CDT-2是在丝状真菌粗神经孢子菌中发现的两种纤维素糊精转运蛋白。以往的研究主要集中在描述这些转运蛋白在少数条件下的作用，包括纤维素降解，这两种转运蛋白的功能尚未完全了解。在这项研究中，我们发现cdt-2的缺失，而不是cdt-1的缺失，不仅会导致Avicel的生长缺陷，也会导致木聚糖的生长缺陷。Cdt-2可以被木聚糖高度诱导，并且该突变体具有木糊精消耗缺陷。cdt-2缺失菌株在Avicel和木聚糖上的转录组学分析表明，cdt-2缺失菌株的主要纤维素酶和半纤维素酶基因显著下调，人工过表达cdt-2增加了粗草中纤维素酶和半纤维素酶的产量。综上所述，这些数据清楚地表明CDT-2在半纤维素的感知和利用中起着关键作用。这是糖转运蛋白首次在半纤维素降解途径中发挥作用。此外，我们发现转录因子XLR-1是cdt-2的主要调控因子，而cdt-1主要受CLR-1调控。这些结果加深了我们对两种纤维素糊精转运蛋白，特别是CDT-2的功能的理解。本研究还为草霉对半纤维素的感知和利用机制提供了新的见解，并可能适用于其他纤维素水解丝状真菌。
CDT-1 and CDT-2 are two cellodextrin transporters discovered in the filamentous fungus Neurospora crassa. Previous studies focused on characterizing the role of these transporters in only a few conditions, including cellulose degradation, and the function of these two transporters is not yet completely understood. In this study, we show that deletion of cdt-2, but not cdt-1, results in growth defects not only on Avicel but also on xylan. cdt-2 can be highly induced by xylan, and this mutant has a xylodextrin consumption defect. Transcriptomic analysis of the cdt-2 deletion strain on Avicel and xylan showed that major cellulase and hemicellulase genes were significantly down-regulated in the cdt-2 deletion strain and artificial over expression of cdt-2 in N. crassa increased cellulase and hemicellulase production. Together, these data clearly show that CDT-2 plays a critical role in hemicellulose sensing and utilization. This is the first time a sugar transporter has been assigned a function in the hemicellulose degradation pathway. Furthermore, we found that the transcription factor XLR-1 is the major regulator of cdt-2, while cdt-1 is primarily regulated by CLR-1. These results deepen our understanding of the functions of both cellodextrin transporters, particularly for CDT-2. Our study also provides novel insight into the mechanisms for hemicellulose sensing and utilization in N. crassa, and may be applicable to other cellulolytic filamentous fungi.
登录
查看更多内容
影响因子：
3.7
作者：
Ivanova C;Bååth JA;Seiboth B;Kubicek CP
通讯作者：
Kubicek CP
影响因子：
8
作者：
Marguerat, Samuel;Baehler, Juerg
通讯作者：
Baehler, Juerg
DOI：
10.1073/pnas.1010456108
发表时间：
2011-01-11
影响因子：
11.1
作者：
Ha, Suk-Jin;Galazka, Jonathan M.;Jin, Yong-Su
通讯作者：
Jin, Yong-Su
影响因子：
2.1
作者：
PINAGA, F;FERNANDEZESPINAR, MT;RAMON, D
通讯作者：
RAMON, D
DOI：
10.1073/pnas.1200785109
发表时间：
2012-05-08
影响因子：
11.1
作者：
Coradetti, Samuel T.;Craig, James P.;Glass, N. Louise
通讯作者：
Glass, N. Louise