Psoralea corylifolia L. Attenuates Nonalcoholic Steatohepatitis in Juvenile Mouse.
Psoralea corylifolia L. Attenuates Nonalcoholic Steatohepatitis in Juvenile Mouse.
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Psoralea corylifolia L. 减轻幼年小鼠的非酒精性脂肪性肝炎。
DOI：
10.3389/fphar.2017.00876
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发表时间：
2017
影响因子：
5.6
通讯作者：
Yan S
中科院分区：
文献类型：
--
作者：
Zhou L;Tang J;Xiong X;Dong H;Huang J;Zhou S;Zhang L;Qin H;Yan S
补骨脂（PC）是一种传统中药，用于治疗小儿脾肾阳虚疾病。最近的研究表明其具有肝脏保护和抗氧化作用。本研究旨在探讨PC对幼年小鼠非酒精性脂肪性肝炎的作用及机制。采用母子高脂饲料喂养建立幼年小鼠非酒精性脂肪肝/非酒精性脂肪性肝炎（NAFLD/NASH）模型。制备PC颗粒并评估其质量。采用高效液相色谱法对主要成分进行了鉴定。然后，给予不同剂量的PC 6周。检查了胰岛素抵抗、血浆肝酶、肝脏形态、肝脏超氧阴离子和甘油三酯/总胆固醇水平的稳态模型评估。还测定了肝组织中核因子-κB（NF-κB）活性磷脂酰肌醇3激酶（PI3K）/蛋白激酶B（Akt）和蛋白激酶C-α（PKC-α）/烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶信号通路的变化。我们的数据表明，PC 显着改善幼年 NAFLD/NASH 小鼠的肝功能障碍、肝脏甘油三酯/总胆固醇积累和胰岛素抵抗。 PC 还可以减轻肝门区的脂肪变性、炎症细胞浸润和纤维增生。此外，PC 还能抑制 NF-κB 的激活和炎症因子 mRNA 的表达，同时增强肝组织中的 PI3K/Akt 信号传导。 PC 还可以降低肝组织中的超氧阴离子水平、NADPH 氧化酶活性以及 p47phox 蛋白表达和 PKCα 激活。结果表明，PC 可有效治疗幼年小鼠 NASH。其机制可能与通过PKC-α/NADPH氧化酶信号通路减轻肝脏氧化应激有关。
Psoralea corylifolia L. (PC) is a traditional Chinese herb used to treat yang deficiency of the spleen and kidney in pediatric disease. Recent studies have shown its liver protection and anti-oxidative effects. The aim of this study was to explore the effect and mechanism of PC on nonalcoholic steatohepatitis in juvenile mice. The juvenile mouse model of nonalcoholic fatty liver disease/nonalcoholic steatohepatitis (NAFLD/NASH) was established by being fed a high-fat diet in maternal-offspring manner. PC granules were prepared and the quality was assessed. The main components were identified by high performance liquid chromatography. Then, different dosages of PC were administered for 6 weeks. Homeostatic model assessment of insulin resistance, plasma liver enzymes, hepatic morphology, hepatic superoxide anion, and triglyceride/total cholesterol levels were examined. The changes of nuclear factor-κB (NF-κB) activity phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt) and protein kinase C-α (PKC-α)/nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase signaling pathways in hepatic tissues were also determined. Our data demonstrated that PC significantly improved liver dysfunction, liver triglyceride/total cholesterol accumulation and insulin resistance in juvenile NAFLD/NASH mice. PC also alleviated hepatic steatosis, inflammatory cell infiltration, and fibroplasia in the portal area. Additionally, PC inhibited the activation of NF-κB and the mRNA expression of inflammatory factors while enhancing PI3K/Akt signaling in hepatic tissues. PC could also reduce hepatic superoxide anion levels, and NADPH oxidase activity as well as p47phox protein expression and PKCα activation in hepatic tissues. The results suggest that PC is effective in the treatment of NASH in juvenile mice. The mechanism may be related to the attenuation of hepatic oxidative stress through the PKC-α/NADPH oxidase signaling pathway.
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影响因子：
5.2
作者：
Pisonero-Vaquero, Sandra;Martinez-Ferreras, Angel;Sanchez-Campos, Sonia
通讯作者：
Sanchez-Campos, Sonia
影响因子：
8.9
作者：
Ji, C. -Y.
通讯作者：
Ji, C. -Y.
影响因子：
3.6
作者：
Guo, Xiaofan;Zheng, Liqiang;Sun, Yingxian
通讯作者：
Sun, Yingxian
中国酒精性和非酒精性脂肪肝流行病学
影响因子：
4.1
作者：
Fan, Jian-Gao
通讯作者：
Fan, Jian-Gao
影响因子：
13.5
作者：
Loomba, Rohit;Sirlin, Claude B.;Schwimmer, Jeffrey B.;Lavine, Joel E.
通讯作者：
Lavine, Joel E.