Regulation of Tyrosine Hydroxylase and Dopamine β‐Hydroxylase mRNA Levels in Rat Adrenals by a Single and Repeated Immobilization Stress
Regulation of Tyrosine Hydroxylase and Dopamine β‐Hydroxylase mRNA Levels in Rat Adrenals by a Single and Repeated Immobilization Stress
复制标题
单次和重复固定应力对大鼠肾上腺酪氨酸羟化酶和多巴胺 β-羟化酶 mRNA 水平的调节
作者：
A. Mcmahon;Richard Kvetňansk;K. Fukuhara;V. Weise;I. Kopin;E. Sabban
翻译后摘要：肾上腺儿茶酚胺是已知的调解许多的生理后果的“战斗或逃跑”的压力反应。然而，介导对重复应激的长期反应的机制还不太清楚，可能涉及基因表达的改变。在这项研究中，检查了单次和重复固定应激对肾上腺儿茶酚胺生物合成酶、酪氨酸羟化酶和多巴胺β-羟化酶mRNA水平的影响。连续7天每天重复2小时的固定显著升高酪氨酸羟化酶和多巴胺β羟化酶mRNA水平（分别约为6倍和4倍）。相反，酪氨酸羟化酶而不是多巴胺β-羟化酶mRNA水平在单次固定后立即升高。酪氨酸羟化酶mRNA与一个单一的固定的海拔高达7天重复固定。这种升高并未持续，24小时后恢复到对照值。酪氨酸羟化酶和多巴胺β-羟化酶mRNA水平在每天两次固定后立即升高至与7次固定后观察到的水平相似的水平，并在24小时后维持。结果表明，酪氨酸羟化酶和多巴胺β-羟化酶的mRNA水平都因应激而升高;然而，它们的调节机制和/或时间并不相同。
Abstract: Adrenal catecholamines are known to mediate many of the physiological consequences of the “fight or flight” response to stress. However, the mechanisms by which the long‐term responses to repeated stress are mediated are less well understood and possibly involve alterations in gene expression. In this study the effects of a single and repeated immobilization stress on mRNA levels of the adrenal catecholamine biosynthetic enzymes, tyrosine hydroxylase and dopamine β‐hydroxylase, were examined. A repeated 2‐hr daily immobilization for 7 consecutive days markedly elevated both tyrosine hydroxylase and dopamine β‐hydroxylase mRNA levels (about six‐ and fourfold, respectively). In contrast, tyrosine hydroxylase but not dopamine β‐hydroxylase mRNA levels were elevated immediately following a single immobilization. The elevation in tyrosine hydroxylase mRNA with a single immobilization was as high as with seven daily repeated immobilizations. This elevation was not sustained and returned toward control values 24 hr later. Both tyrosine hydroxylase and dopamine β‐hydroxylase mRNA levels were elevated immediately following two daily immobilizations to levels similar to those observed after seven immobilizations and were maintained 24 hr later. The results indicate that both tyrosine hydroxylase and dopamine β‐hydroxylase mRNA levels are elevated by stress; however, the mechanism and/or timing of their regulation are not identical.
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DOI：
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--
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DOI：
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影响因子：
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