Spine formation and maturation in the developing rat auditory cortex.
Spine formation and maturation in the developing rat auditory cortex.
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DOI：
10.1002/cne.22728
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发表时间：
2011-11-01
影响因子：
2.5
通讯作者：
Green, Steven H.
中科院分区：
文献类型：
--
作者：
Schachtele, Scott J.;Losh, Joe;Dailey, Michael E.;Green, Steven H.
大鼠听觉皮层被组织为声音频率的音调分布图。这张地图在出生时被广泛调整，并在出生后的前3周内得到完善。发育过程中色调图成熟和重组背后的结构相关性知之甚少。我们采用荧光染料弹道标记（“DiOlistics”）单独，或与免疫组织化学结合，以量化正常听力大鼠的听觉皮层中的突触发生。我们发现，树突状突起的发育外观，其中包括未成熟的丝状伪足和成熟的棘，在第2/3，4和5层锥体和第4层多刺非锥体神经元发生在三个阶段：从出生后第4天（P4）到P9缓慢增加树突状突起，从P9到P19快速增加树突状突起，以及达到成熟突起密度的最终阶段（>P21）。接下来，我们将DiOlistics与巴松管（一种突触前支架蛋白）的免疫组织化学标记相结合，作为一种新方法，将树枝状突起分类为体内固定的皮质中的丝状伪足或成熟棘。使用这种方法，我们观察到的增加，从P9-P16的棘丝状伪足的比例，表明一个时期的快速脊柱成熟。以前的研究报告成熟的刺是较短的长度相比，丝状伪足。我们同样观察到P9和P16之间的突出长度减少，证实了我们的免疫组化脊柱成熟数据。这些研究表明，树突突起的形成和棘成熟迅速发生在一个时间以前显示对应于听觉皮层tonotopic地图细化（P11-P14），提供了一个结构相关的生理成熟。
The rat auditory cortex is organized as a tonotopic map of sound frequency. This map is broadly tuned at birth and is refined during the first 3 weeks postnatal. The structural correlates underlying tonotopic map maturation and reorganization during development are poorly understood. We employed fluorescent dye ballistic labeling (“DiOlistics”) alone, or in conjunction with immunohistochemistry, to quantify synaptogenesis in the auditory cortex of normal hearing rats. We show that the developmental appearance of dendritic protrusions, which include both immature filopodia and mature spines, on layers 2/3, 4, and 5 pyramidal and layer 4 spiny nonpyramidal neurons occurs in three phases: slow addition of dendritic protrusions from postnatal day 4 (P4) to P9, rapid addition of dendritic protrusions from P9 to P19, and a final phase where mature protrusion density is achieved (>P21). Next, we combined DiOlistics with immunohistochemical labeling of bassoon, a presynaptic scaffolding protein, as a novel method to categorize dendritic protrusions as either filopodia or mature spines in cortex fixed in vivo. Using this method we observed an increase in the spine-to-filopodium ratio from P9–P16, indicating a period of rapid spine maturation. Previous studies report mature spines as being shorter in length compared to filopodia. We similarly observed a reduction in protrusion length between P9 and P16, corroborating our immunohistochemical spine maturation data. These studies show that dendritic protrusion formation and spine maturation occur rapidly at a time previously shown to correspond to auditory cortical tonotopic map refinement (P11–P14), providing a structural correlate of physiological maturation.
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影响因子：
3.3
作者：
Franceschetti, S;Sancini, G;Avanzini, G
通讯作者：
Avanzini, G
DOI：
10.1007/bf00522528
发表时间：
1969-01-01
期刊：
ZEITSCHRIFT FUR ANATOMIE UND ENTWICKLUNGSGESCHICHTE
影响因子：
--
作者：
MOREST, DK
通讯作者：
MOREST, DK
影响因子：
16.2
作者：
Gan, WB;Grutzendler, J;Lichtman, JW
通讯作者：
Lichtman, JW
影响因子：
2.5
作者：
GERMROTH, PG;GOURDIE, RG;THOMPSON, RP
通讯作者：
THOMPSON, RP
影响因子：
4.8
作者：
Hrabovszky, E;Turi, GF;Liposits, Z
通讯作者：
Liposits, Z