Co-fermentation of cellobiose and xylose by mixed culture of recombinant Saccharomyces cerevisiae and kinetic modeling.
Co-fermentation of cellobiose and xylose by mixed culture of recombinant Saccharomyces cerevisiae and kinetic modeling.
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通过重组酵母酿酒酵母和动力学建模的混合培养物将纤维素和木糖共同发酵。
DOI：
10.1371/journal.pone.0199104
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Wei N
中科院分区：
文献类型：
--
作者：
Chen Y;Wu Y;Zhu B;Zhang G;Wei N
纤维素水解产物中纤维素糖的有效转化对于从木质纤维素生物质经济可行地生产生物燃料是重要的，但该目标仍然是一个关键的挑战。本研究报道了一种利用重组酿酒酵母专业菌株组成的共培养物同时发酵纤维二糖和木糖的新方法。与单一培养系统相比，共培养系统可以提供模块化的竞争优势，并且可以进行调整以处理原料组成的波动，从而实现稳健且具有成本效益的生物燃料生产。本研究对重组纤维二糖消耗型S.酿酒酵母菌株EJ 2、木糖消耗型S.酿酒酵母菌株SR 8，以及它们的共培养物。动力学建模的动机是提供指导和预测使用共培养系统的混合糖的同时发酵与可调的生物量的每个专业菌株在不同的底物浓度。基于纯培养物模型建立了共培养体系的动力学模型，并考虑了产物抑制、初始底物浓度和接种量的影响。通过不同底物条件下的独立发酵实验结果验证了模型模拟的有效性，纤维二糖、木糖及其混合物的分批发酵实验数据与模型预测值吻合较好。此外，在模型预测的指导下，EJ 2和SR 8分别以0.45 g干重/L和0.9 g干重/L的初始细胞密度实现了60 g/L纤维二糖和20 g/L木糖的同时共发酵。结果表明，动力学模型可用于指导酵母共培养条件的设计和优化，以实现纤维二糖和木糖的同时发酵，并提高乙醇产率，这对于从木质纤维素生物质中稳健高效地生产可再生生物燃料至关重要。
Efficient conversion of cellulosic sugars in cellulosic hydrolysates is important for economically viable production of biofuels from lignocellulosic biomass, but the goal remains a critical challenge. The present study reports a new approach for simultaneous fermentation of cellobiose and xylose by using the co-culture consisting of recombinant Saccharomyces cerevisiae specialist strains. The co-culture system can provide competitive advantage of modularity compared to the single culture system and can be tuned to deal with fluctuations in feedstock composition to achieve robust and cost-effective biofuel production. This study characterized fermentation kinetics of the recombinant cellobiose-consuming S. cerevisiae strain EJ2, xylose-consuming S. cerevisiae strain SR8, and their co-culture. The motivation for kinetic modeling was to provide guidance and prediction of using the co-culture system for simultaneous fermentation of mixed sugars with adjustable biomass of each specialist strain under different substrate concentrations. The kinetic model for the co-culture system was developed based on the pure culture models and incorporated the effects of product inhibition, initial substrate concentration and inoculum size. The model simulations were validated by results from independent fermentation experiments under different substrate conditions, and good agreement was found between model predictions and experimental data from batch fermentation of cellobiose, xylose and their mixtures. Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8. The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.
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影响因子：
6.3
作者：
Chen Y;Sheng J;Jiang T;Stevens J;Feng X;Wei N
通讯作者：
Wei N
DOI：
10.1073/pnas.1010456108
发表时间：
2011-01-11
影响因子：
11.1
作者：
Ha, Suk-Jin;Galazka, Jonathan M.;Jin, Yong-Su
通讯作者：
Jin, Yong-Su
影响因子：
5.1
作者：
Bobadilla Fazzini, Roberto A.;Preto, Maria J.;Martins dos Santos, Vitor A. P.
通讯作者：
Martins dos Santos, Vitor A. P.
影响因子：
5
作者：
Katahira, Satoshi;Mizuike, Atsuko;Kondo, Akihiko
通讯作者：
Kondo, Akihiko
影响因子：
5
作者：
Hanly, Timothy J.;Urello, Morgan;Henson, Michael A.
通讯作者：
Henson, Michael A.