The first crop plant genetically engineered to release an insect pheromone for defence.
The first crop plant genetically engineered to release an insect pheromone for defence.
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第一个作物植物在基因上设计了用于防御的昆虫信息素。
DOI：
10.1038/srep11183
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发表时间：
2015-06-25
影响因子：
4.6
通讯作者：
Pickett JA
中科院分区：
文献类型：
--
作者：
Bruce TJA;Aradottir GI;Smart LE;Martin JL;Caulfield JC;Doherty A;Sparks CA;Woodcock CM;Birkett MA;Napier JA;Jones HD;Pickett JA
昆虫信息素为控制作物害虫提供了潜力。挥发性和不稳定性是农业中部署的问题，但可以通过在作物植物中表达用于信息素生物合成的基因来解决。这已经通过遗传工程改造小麦的六倍体品种来实现，以释放（E）-β-法呢烯（Eβf），许多害虫蚜虫的警报信息素，使用基于具有质体靶向氨基酸序列的薄荷序列的合成基因，具有或不具有用于前体法呢基二磷酸生物合成的基因。纯Eβf在稳定转化的小麦品系中产生，没有其他可检测的表型，但需要将产生的基因靶向质体。在实验室行为测定中，三种谷物蚜虫被击退，觅食增加了寄生性天敌。尽管这些研究显示出相当大的蚜虫控制潜力，但采用单构建体和双构建体的田间试验显示蚜虫没有减少或寄生率没有增加。昆虫数量低，气候条件不稳定，表明需要进一步试验或更密切的模仿，在工厂，报警信息素释放。
Insect pheromones offer potential for managing pests of crop plants. Volatility and instability are problems for deployment in agriculture but could be solved by expressing genes for the biosynthesis of pheromones in the crop plants. This has now been achieved by genetically engineering a hexaploid variety of wheat to release (E)-β-farnesene (Eβf), the alarm pheromone for many pest aphids, using a synthetic gene based on a sequence from peppermint with a plastid targeting amino acid sequence, with or without a gene for biosynthesis of the precursor farnesyl diphosphate. Pure Eβf was produced in stably transformed wheat lines with no other detectable phenotype but requiring targeting of the gene produced to the plastid. In laboratory behavioural assays, three species of cereal aphids were repelled and foraging was increased for a parasitic natural enemy. Although these studies show considerable potential for aphid control, field trials employing the single and double constructs showed no reduction in aphids or increase in parasitism. Insect numbers were low and climatic conditions erratic suggesting the need for further trials or a closer imitation, in the plant, of alarm pheromone release.
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DOI：
10.1073/pnas.1208756109
发表时间：
2012-12-04
影响因子：
11.1
作者：
Bleeker, Petra M.;Mirabella, Rossana;Schuurink, Robert C.
通讯作者：
Schuurink, Robert C.
影响因子：
2.3
作者：
Al Abassi, S;Birkett, MA;Woodcock, CM
通讯作者：
Woodcock, CM
影响因子：
2.3
作者：
Webster, Ben;Bruce, Toby;Pickett, John
通讯作者：
Pickett, John
影响因子：
3.8
作者：
Prosser, Ian M.;Adams, Racheal J.;Field, Linda M.
通讯作者：
Field, Linda M.
影响因子：
4.1
作者：
Bruce, TJA;Birkett, MA;Wadhams, LJ
通讯作者：
Wadhams, LJ