Fungal Gene Mutation Analysis Elucidating Photoselective Enhancement of UV-C Disinfection Efficiency Toward Spoilage Agents on Fruit Surface.
Fungal Gene Mutation Analysis Elucidating Photoselective Enhancement of UV-C Disinfection Efficiency Toward Spoilage Agents on Fruit Surface.
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真菌基因突变分析阐明 UV-C 对水果表面腐败剂的光选择性增强消毒效率
DOI：
10.3389/fmicb.2018.01141
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发表时间：
2018
影响因子：
5.2
通讯作者：
Xu L
中科院分区：
文献类型：
--
作者：
Zhu P;Li Q;Azad SM;Qi Y;Wang Y;Jiang Y;Xu L
短波紫外线（UV-C）处理是一种有效、清洁和安全的化学消毒剂替代品，用于保鲜水果。然而，微生物消毒的用量要求可能会对水果品质产生负面影响。在本研究中，与白光和蓝光相比，UV-C在暗光和红光条件下对灰葡萄孢的杀灭效率更高。蓝光受体基因Bcwcl1的缺失导致在所有光照条件下对UV-C过敏。Bcwcl1是粗神经孢子虫中wc-1的同源基因。白光和蓝光强烈诱导Bcuve1和Bcphr1的表达，Bcuve1和Bcphr1分别编码紫外线损伤内切酶和光解酶，且表达依赖bcwcl1。Bcuve1和Bcphr1的基因突变分析表明，它们协同促进UV-C治疗后的生存。体内实验表明，UV-C （1.0 kJ/m2）仅在UV-C处理后的黑暗期或红光下才能消除水滴接种果实的腐烂，而UV-C照射后的果实在白光或蓝光下则出现典型的腐烂。综上所述，蓝光通过诱导紫外线损伤修复相关酶的表达增强了灰葡萄对UV- c的抗性，而在暗光或红光条件下可以提高UV- c对果实表面消毒的效率；这些原则似乎在采后真菌病原体中得到很好的保存。
Short-wave ultraviolet (UV-C) treatment represents a potent, clean and safe substitute to chemical sanitizers for fresh fruit preservation. However, the dosage requirement for microbial disinfection may have negative effects on fruit quality. In this study, UV-C was found to be more efficient in killing spores of Botrytis cinerea in dark and red light conditions when compared to white and blue light. Loss of the blue light receptor gene Bcwcl1, a homolog of wc-1 in Neurospora crassa, led to hypersensitivity to UV-C in all light conditions tested. The expression of Bcuve1 and Bcphr1, which encode UV-damage endonuclease and photolyase, respectively, were strongly induced by white and blue light in a Bcwcl1-dependent manner. Gene mutation analyses of Bcuve1 and Bcphr1 indicated that they synergistically contribute to survival after UV-C treatment. In vivo assays showed that UV-C (1.0 kJ/m2) abolished decay in drop-inoculated fruit only if the UV-C treatment was followed by a dark period or red light, while in contrast, typical decay appeared on UV-C irradiated fruits exposed to white or blue light. In summary, blue light enhances UV-C resistance in B. cinerea by inducing expression of the UV damage repair-related enzymes, while the efficiency of UV-C application for fruit surface disinfection can be enhanced in dark or red light conditions; these principles seem to be well conserved among postharvest fungal pathogens.
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影响因子：
56.9
作者：
Crosthwaite, SK;Dunlap, JC;Loros, JJ
通讯作者：
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