Cellular localization of Y-box binding protein 1 in brain tissue of rats, macaques, and humans.
Cellular localization of Y-box binding protein 1 in brain tissue of rats, macaques, and humans.
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DOI：
10.1186/1471-2202-10-28
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发表时间：
2009-03-26
期刊：
影响因子：
2.4
通讯作者：
Potschka H
中科院分区：
文献类型：
--
作者：
Unkrüer B;Pekcec A;Fuest C;Wehmeyer A;Balda MS;Horn A;Baumgärtner W;Potschka H
Y-box结合蛋白1（YB-1）被认为是转录和翻译的关键调节因子之一。然而，到目前为止，关于其在成人大脑中的细胞分布的知识有限。YB-1免疫标记以及双标记的神经元标记NeuN在大鼠脑组织中的分析显示，在齿状回，角氨锥体细胞层，第三层的梨状皮质，以及整个海马旁皮质的所有层的主要神经元的表达。在海马门区，单个神经元表达YB-1。成年猕猴和人类的海马和海马旁皮质中的神经元表达模式相当。YB-1与内皮细胞标记物Glut-1、多药转运蛋白P-糖蛋白和星形胶质细胞标记物GFAP的双标记未表明共定位。在大鼠癫痫持续状态后，脑毛细血管内皮细胞和神经元中没有YB-1的诱导。总之，我们的研究表明，YB-1主要表达在成年大鼠，猕猴和人类大脑的神经元中。缺乏与Glut-1和P-糖蛋白的共定位反对YB-1在血脑屏障P-糖蛋白调节中的直接作用。
The Y-box binding protein 1 (YB-1) is considered to be one of the key regulators of transcription and translation. However, so far only limited knowledge exists regarding its cellular distribution in the adult brain. Analysis of YB-1 immunolabelling as well as double-labelling with the neuronal marker NeuN in rat brain tissue revealed a predominant neuronal expression in the dentate gyrus, the cornu ammonis pyramidal cell layer, layer III of the piriform cortex as well as throughout all layers of the parahippocampal cortex. In the hilus of the hippocampus single neurons expressed YB-1. The neuronal expression pattern was comparable in the hippocampus and parahippocampal cortex of adult macaques and humans. Double-labelling of YB-1 with the endothelial cell marker Glut-1, the multidrug transporter P-glycoprotein, and the astrocytic marker GFAP did not indicate a co-localization. Following status epilepticus in rats, no induction of YB-1 occurred in brain capillary endothelial cells and neurons. In conclusion, our study demonstrates that YB-1 is predominantly expressed in neurons in the adult brain of rats, macaques and humans. Lack of a co-localization with Glut-1 and P-glycoprotein argues against a direct role of YB-1 in the regulation of blood-brain barrier P-glycoprotein.
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