High-throughput microbead assay system with a portable, cost-effective Wi-Fi imaging module, and disposable multi-layered microfluidic cartridges for virus and microparticle detection, and tracking.
High-throughput microbead assay system with a portable, cost-effective Wi-Fi imaging module, and disposable multi-layered microfluidic cartridges for virus and microparticle detection, and tracking.
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DOI：
10.1007/s10544-023-00661-3
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发表时间：
2023-06-07
影响因子：
2.8
通讯作者：
Rajaraman, Swaminathan
中科院分区：
文献类型：
--
作者：
Castro, Jorge Manrique;Sommerhage, Frank;Khanna, Rishika;Childs, Andre;DeRoo, David;Rajaraman, Swaminathan
近年来，生物医学科学界一直致力于开发高通量设备，使其能够同时可靠、快速和平行地检测多种病毒株或微粒。这一问题的复杂性之一在于新设备的快速原型制作以及对小颗粒和病毒的无线快速检测。通过降低微流体微加工的复杂性和使用经济材料以及制造商空间工具（Kundu等人），有可能为高通量设备和检测技术的问题提供经济实惠的解决方案。我们提出了一种无线、独立设备和一次性微流控芯片的开发，基于电动和非电动微珠检测，以及这些微珠以微米为单位的运动轨迹的成像处理，可以快速地从鼻腔或唾液样本中产生选定的可能的病毒变体的并行读出。对微珠和SARS-CoV-2 COVID-19 δ变体进行了测试，作为测试微流体盒和无线成像模块的概念验证。Microbead Assay （MA）系统套件包括一个Wi-Fi读出模块、一个微流控芯片和一个样品采集/处理子系统。在这里，我们专注于微流控芯片的制造和表征，以复用各种微米大小的珠子，以便在一次测试中同时检测多达六种不同的病毒、微粒或变体，并使用市售的、具有wi - fi功能的和相机集成设备进行数据收集（图1）。在线版本包含补充材料，可在10.1007/s10544-023-00661-3获得。
In recent years biomedical scientific community has been working towards the development of high-throughput devices that allow a reliable, rapid and parallel detection of several strains of virus or microparticles simultaneously. One of the complexities of this problem lies on the rapid prototyping of new devices and wireless rapid detection of small particles and virus alike. By reducing the complexity of microfluidics microfabrication and using economic materials along with makerspace tools (Kundu et al.) it is possible to provide an affordable solution to both the problems of high-throughput devices and detection technologies. We present the development of a wireless, standalone device and disposable microfluidics chips that rapidly generate parallel readouts for selected, possible virus variants from a nasal or saliva sample, based on motorized and non-motorized microbeads detection, and imaging processing of the motion tracks of these beads in micrometers. Microbeads and SARS-CoV-2 COVID-19 Delta variant were tested as proof-of-concept for testing the microfluidic cartridges and wireless imaging module. The Microbead Assay (MA) system kit consists of a Wi-Fi readout module, a microfluidic chip, and a sample collection/processing sub-system. Here, we focus on the fabrication and characterization of the microfluidic chip to multiplex various micrometer-sized beads for economic, disposable, and simultaneous detection of up to six different viruses, microparticles or variants in a single test, and data collection using a commercially available, Wi-Fi-capable, and camera integrated device (Fig. 1). The online version contains supplementary material available at 10.1007/s10544-023-00661-3.
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