Impact of Menthol on Growth and Photosynthetic Function of Breviolum Minutum (Dinoflagellata, Dinophyceae, Symbiodiniaceae) and Interactions with its Aiptasia Host
Impact of Menthol on Growth and Photosynthetic Function of Breviolum Minutum (Dinoflagellata, Dinophyceae, Symbiodiniaceae) and Interactions with its Aiptasia Host
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薄荷醇对短叶藻（甲藻、甲藻纲、共生藻科）生长和光合功能的影响及其与其寄主的相互作用
DOI：
10.1111/jpy.13081
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发表时间：
2021
影响因子：
2.9
通讯作者：
Grossman, Arthur R.
中科院分区：
文献类型：
--
作者：
Clowez, Sophie;Renicke, Christian;Pringle, John R.;Grossman, Arthur R.
环境变化，包括全球变暖和化学污染，可以损害刺胞菌-(例如：（珊瑚）鞭毛藻共生并导致珊瑚白化。了解调节这些共生的机制将为维持健康的珊瑚礁群落提供策略。珊瑚的一个模式系统是海葵（exaiptasia pallida）和它的甲藻伙伴（symbiodiiaceae家族）之间的共生关系。为了补充这些生物之间相互作用的现有研究，我们研究了薄荷醇的影响，薄荷醇是一种通常用于使刺胞菌非共生的试剂，在培养和招待所中对鞭毛菌breviolum minutum的影响。在这两种环境中，这种藻类的生长和光合作用受到100或300µM薄荷醇的影响。我们观察到PSII和PSI功能的降低，反应中心蛋白的丰度，以及在300µM薄荷醇下细胞总蛋白的减少。有趣的是，对于暴露于100 μ M薄荷醇的自由生活藻类，生长、光合活性、色素沉着和蛋白质丰度的最初下降在5-15 d后逆转，最终接近控制水平。这种行为在持续光照下的细胞中被观察到，但在经历光-暗方案的细胞中没有观察到，这表明b。minutum可以通过光和/或能量依赖的方式解毒薄荷醇或适应和修复受损的光合复合体。将培养藻类长期暴露于300µM薄荷醇中最终导致藻类死亡。大多数共生海葵也无法在这种薄荷醇浓度下存活30天。此外，被300µM薄荷醇预处理的光合作用受损的细胞在重新繁殖海葵宿主时表现出降低的效率。
Environmental change, including global warming and chemical pollution, can compromise cnidarian‐(e.g., coral‐) dinoflagellate symbioses and cause coral bleaching. Understanding the mechanisms that regulate these symbioses will inform strategies for sustaining healthy coral–reef communities. A model system for corals is the symbiosis between the sea anemoneExaiptasia pallida(common nameAiptasia) and its dinoflagellate partners (family Symbiodiniaceae). To complement existing studies of the interactions between these organisms, we examined the impact of menthol, a reagent often used to render cnidarians aposymbiotic, on the dinoflagellateBreviolum minutum, both in culture and in hospite. In both environments, the growth and photosynthesis of this alga were compromised at either 100 or 300 µM menthol. We observed reduction in PSII and PSI functions, the abundances of reaction‐center proteins, and, at 300 µM menthol, of total cellular proteins. Interestingly, for free‐living algae exposed to 100 µM menthol, an initial decline in growth, photosynthetic activities, pigmentation, and protein abundances reversed after 5–15 d, eventually approaching control levels. This behavior was observed in cells maintained in continuous light, but not in cells experiencing a light–dark regimen, suggesting thatB. minutumcan detoxify menthol or acclimate and repair damaged photosynthetic complexes in a light‐ and/or energy‐dependent manner. Extended exposures of cultured algae to 300 µM menthol ultimately resulted in algal death. Most symbiotic anemones were also unable to survive this menthol concentration for 30 d. Additionally, cells impaired for photosynthesis by pre‐treatment with 300 µM menthol exhibited reduced efficiency in re‐populating the anemone host.
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影响因子：
2.9
作者：
Xiang, Tingting;Hambleton, Elizabeth A.;Grossman, Arthur R.
通讯作者：
Grossman, Arthur R.
珊瑚Siderastrea弧度及其幼体发育。
影响因子：
4.9
作者：
J. E. Duerden
通讯作者：
J. E. Duerden
影响因子：
4.1
作者：
Samarasekera, Radhika;Weerasinghe, Indira S.;Hemalal, K. D. Patrick
通讯作者：
Hemalal, K. D. Patrick
影响因子：
2.7
作者：
Wang JT;Keshavmurthy S;Chu TY;Chen CA
通讯作者：
Chen CA
光合作用抑制剂对珊瑚和其他海洋无脊椎动物中虫黄藻的影响
影响因子：
2.4
作者：
J. Vandermeulen;N. Davis;L. Muscatine
通讯作者：
L. Muscatine