A dynamin-related protein required for nuclear remodeling in Tetrahymena.
A dynamin-related protein required for nuclear remodeling in Tetrahymena.
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DOI：
10.1016/j.cub.2008.07.042
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发表时间：
2008-08-26
期刊：
影响因子：
9.2
通讯作者：
Turkewitz, Aaron P.
中科院分区：
文献类型：
--
作者：
Rahaman, Abdur;Elde, Nels C.;Turkewitz, Aaron P.
动力蛋白相关蛋白（DRPs）是在细胞膜上可逆组装的GTPases。单个DRP（为简单起见，我们使用“DRP”来包括真正的发动蛋白）在质膜、高尔基体网络、线粒体、过氧化物酶体、叶绿体和内体的分裂或变性中以及在线粒体融合中起作用。这些功能中的许多是广泛存在的，存在于动物、植物、锥虫、贾第虫、纤毛虫、寄生虫和黏菌中。该基因家族的谱系特异性扩增在动物中产生了包括MxB在内的专门的DRP，据报道可调节核孔运输。虽然许多单细胞生物拥有少量的DRP，但在一些原生生物谱系中发生了扩张。四膜虫中的8个DRPs可能对纤毛虫的复杂性有贡献。每一个纤毛虫细胞都有不同的生殖细胞核和体细胞核，它们的分化和维持需要不同的机制。在这里，我们表明，Drp6p，以前被证明是针对核膜，是所需的大核（mac）的发展。Drp6p的活性，这是唯一的其他已知的核DRP不同，是由特定阶段的亚细胞靶向和组装动力学的组合调制。这项工作展示了一种新的活动的DRP，并提出了一个系统，其中的关键方面的监管可以很容易地操纵环境和发展的线索。
Dynamin-related proteins (DRPs) are GTPases that reversibly assemble on cellular membranes. Individual DRPs (for simplicity, we use “DRP” to include authentic dynamins) function in fission or tubulation of the plasma membrane, trans-Golgi network, mitochondria, peroxisomes, chloroplasts, and endosomes, and in mitochondrial fusion. Many of these functions are widespread, present in animals, plants, trypanosomes, Giardia, ciliates, alga, and slime molds. Lineage-specific expansions of the gene family created specialized DRPs including MxB in animals, reported to regulate nuclear pore transport. While many unicellular organisms possess a small number of DRPs, expansions occurred in some protist lineages. The 8 DRPs in the ciliate Tetrahymena thermophila may contribute to aspects of ciliate complexity. Each ciliate cell contains distinct germ line and somatic nuclei, which must require distinct machinery for their differentiation and maintenance. Here we show that Drp6p, previously shown to be targeted to the nuclear envelope, is required for macronuclear (mac) development. Drp6p activity, which is distinct from that of the only other known nuclear DRP, is modulated by a combination of stage-specific subcellular targeting and assembly dynamics. This work demonstrates a novel activity for a DRP, and presents a system in which key aspects of regulation can be easily manipulated by environmental and developmental cues.
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DOI：
10.1073/pnas.0403167101
发表时间：
2004-06-15
影响因子：
11.1
作者：
King, MC;Raposo, G;Lemmon, MA
通讯作者：
Lemmon, MA
DOI：
10.1073/pnas.0436886100
发表时间：
2003-02-18
影响因子：
11.1
作者：
Nishida, K;Takahara, M;Kuroiwa, T
通讯作者：
Kuroiwa, T
影响因子：
9.2
作者：
Harder, Z;Zunino, R;McBride, H
通讯作者：
McBride, H
影响因子：
2.7
作者：
Ward, JG;Herrick, G
通讯作者：
Herrick, G
DOI：
10.1083/jcb.122.3.553
发表时间：
1993-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
van der Bliek AM;Redelmeier TE;Damke H;Tisdale EJ;Meyerowitz EM;Schmid SL
通讯作者：
Schmid SL