A visual and "turn-on" fluorescent probe for rapid detection of cysteine over homocysteine and glutathione
A visual and "turn-on" fluorescent probe for rapid detection of cysteine over homocysteine and glutathione
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一种视觉和“开启”荧光探针，用于快速检测半胱氨酸、同型半胱氨酸和谷胱甘肽
DOI：
10.1016/j.snb.2014.02.052
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发表时间：
2014-06
期刊：
影响因子：
--
通讯作者：
Yin, Bingzhu
中科院分区：
文献类型：
--
作者：
Li, Hongda;Jin, Longyi;Kan, Yuhe;Yin, Bingzhu
以苯并噻唑-喹啉半菁染料为基料，以丙烯酸酯基为功能触发基团，设计并合成了一种新型生物硫醇化学剂量荧光探针1。探针1在水环境中快速检测半胱氨酸（Cys）比同型半胱氨酸和谷胱甘肽具有高灵敏度和高选择性。在1等量Cys存在的情况下，在1 min内立即达到最大的荧光响应，并且在纳米水平上可以直观地识别感知过程的颜色变化。探针1对Cys具有很高的反应活性和特异响应，主要是由于感应产物苯酚阴离子的稳定性和感应反应动力学的差异。此外，探针1成功地检测了蛋白质中的游离Cys残基，并通过荧光成像监测活HeLa细胞中的Cys。
A novel chemodosimetric fluorescent probe 1 for biothiols was designed and synthesized based on benzothiazolium-quinoline hemicyanine dye with an acrylate group as a functional trigger moiety. Prober 1 exhibited high sensitivity and high selectivity for rapid detection of cysteine (Cys) over homocysteine and glutathione in aqueous environments. The maximal fluorescent response was immediately achieved within 1 min in the presence of only 1 equiv. of Cys, and the color change of the sensing process was discerned visually at the nanomole level. The exceptional high reactivity and specific response of probe 1 for Cys were promoted by the stabilization of the sensing product phenolate anion and the differences in the kinetics of the sensing reaction. Moreover, probe 1 was employed successfully to detect the free Cys residues within protein and monitor Cys in living HeLa cells by fluorescence imaging.
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影响因子：
4.8
作者：
Chiku, Taurai;Padovani, Dominique;Banerjee, Ruma
通讯作者：
Banerjee, Ruma
影响因子：
4.5
作者：
Liguo Wang;Qi Zhou;Baocun Zhu;Liang-guo Yan;Zhenmin Ma;B. Du;Xiaoling Zhang
通讯作者：
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影响因子：
15
作者：
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通讯作者：
Huang, Chunhui
DOI：
10.1002/chin.201213278
发表时间：
2012-03
期刊：
ChemInform
影响因子：
--
作者：
Ying Zhou;Juyoung Yoon
通讯作者：
Ying Zhou;Juyoung Yoon
影响因子：
4.9
作者：
Yongqian Xu;M. Panzner;Xiaopeng Li;W. Youngs;Y. Pang
通讯作者：
Yongqian Xu;M. Panzner;Xiaopeng Li;W. Youngs;Y. Pang