Regulation of transcription by the Arabidopsis UVR8 photoreceptor involves a specific histone modification.
Regulation of transcription by the Arabidopsis UVR8 photoreceptor involves a specific histone modification.
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DOI：
10.1007/s11103-016-0522-3
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发表时间：
2016-11
影响因子：
5.1
通讯作者：
Jenkins, Gareth I.
中科院分区：
文献类型：
--
作者：
Velanis, Christos N.;Herzyk, Pawel;Jenkins, Gareth I.
光感受器UV抗性基因座8（UVR 8）特异性地介导对UV-B波长的光形态发生反应。UVR 8通过调节一组基因的转录发挥作用，但其潜在机制尚不清楚。以往的研究表明，UVR 8可以与染色质结合，但这种结合的特异性和功能意义尚不清楚。在这里，我们表明，通过染色质免疫沉淀，UV-B暴露的拟南芥增加乙酰化的赖氨酸K9和/或K14的组蛋白H3在UVR 8调节基因位点的UVR 8依赖的方式。转录因子HY 5和/或HYH介导UVR 8调节的转录，也是这种染色质修饰所必需的，至少对于ELIP 1基因是如此。此外，免疫沉淀DNA的测序显示，所有UV-B诱导的H3 K9，14双乙酰化在整个基因组中的富集都是UVR 8依赖性的，并且大约40%的富集位点含有已知的UVR 8调节基因。此外，漆树酸对组蛋白乙酰化的抑制降低了UV-B诱导的、UVR 8介导的ELIP 1和CHS表达。没有证据表明，在酵母双杂交试验的直接相互作用之间的UVR 8或HY 5和几个蛋白质参与光调节组蛋白修饰，也没有参与这些蛋白质在UVR 8介导的反应在植物中，虽然蛋白质之间的功能冗余可能会影响结果。总之，这项研究表明，UVR 8调节一个特定的染色质修饰与一组UVR 8靶基因的转录调控。本文的在线版本（doi：10.1007/s11103-016-0522-3）包含补充材料，可供授权用户使用。
The photoreceptor UV RESISTANCE LOCUS 8 (UVR8) specifically mediates photomorphogenic responses to UV-B wavelengths. UVR8 acts by regulating transcription of a set of genes, but the underlying mechanisms are unknown. Previous research indicated that UVR8 can associate with chromatin, but the specificity and functional significance of this interaction are not clear. Here we show, by chromatin immunoprecipitation, that UV-B exposure of Arabidopsis increases acetylation of lysines K9 and/or K14 of histone H3 at UVR8-regulated gene loci in a UVR8-dependent manner. The transcription factors HY5 and/or HYH, which mediate UVR8-regulated transcription, are also required for this chromatin modification, at least for the ELIP1 gene. Furthermore, sequencing of the immunoprecipitated DNA revealed that all UV-B-induced enrichments in H3K9,14diacetylation across the genome are UVR8-dependent, and approximately 40 % of the enriched loci contain known UVR8-regulated genes. In addition, inhibition of histone acetylation by anacardic acid reduces the UV-B induced, UVR8 mediated expression of ELIP1 and CHS. No evidence was obtained in yeast 2-hybrid assays for a direct interaction between either UVR8 or HY5 and several proteins involved in light-regulated histone modification, nor for the involvement of these proteins in UVR8-mediated responses in plants, although functional redundancy between proteins could influence the results. In summary, this study shows that UVR8 regulates a specific chromatin modification associated with transcriptional regulation of a set of UVR8-target genes. The online version of this article (doi:10.1007/s11103-016-0522-3) contains supplementary material, which is available to authorized users.
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DOI：
10.1073/pnas.1503512112
发表时间：
2015-05-26
影响因子：
11.1
作者：
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