Chinese Herbal Formulas Si-Wu-Tang and Er-Miao-San Synergistically Ameliorated Hyperuricemia and Renal Impairment in Rats Induced by Adenine and Potassium Oxonate
Chinese Herbal Formulas Si-Wu-Tang and Er-Miao-San Synergistically Ameliorated Hyperuricemia and Renal Impairment in Rats Induced by Adenine and Potassium Oxonate
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中药方四物汤和二妙散协同改善腺嘌呤和氧酸钾所致大鼠高尿酸血症和肾损伤
DOI：
10.1159/000438517
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发表时间：
2015-10
期刊：
影响因子：
--
通讯作者：
Wang N
中科院分区：
文献类型：
--
作者：
Guo Y;Jiang Q;Gui D;Wang N
背景/目的：高尿酸血症是慢性肾脏病和心血管疾病的独立危险因素。在此，我们研究了中药方四物汤和二妙散对大鼠高尿酸血症和肾功能损害的联合保护作用。方法：将大鼠随机分为正常大鼠、高尿酸血症大鼠和高尿酸血症大鼠，口服苯溴马隆（4.5 mg·kg-1·d-1）、四物汤（3.78 g·kg-1·d-1）和四物汤加二妙散（6.48 g·kg-1·d-1），连续4周。高尿酸血症大鼠每天口服腺嘌呤（0.1 g·kg-1·d-1）和氧酸钾（1.5 g·kg-1·d-1），持续4周。每周测量血清尿酸、肌酐、总胆固醇（TCH）、甘油三酯和血尿素氮（BUN）浓度，以及尿尿酸和微量白蛋白尿。还评估了血清黄嘌呤氧化酶（XOD）活性和肾脏组织病理学。 Western blot检测肾组织有机阴离子转运蛋白1（OAT1）和有机阴离子转运蛋白3（OAT3）的表达。结果：四物汤联合二妙散较单用四物汤更能显着降低血清尿酸、肌酐、甘油三酯和 BUN 水平。四物汤加二妙散可改善高尿酸血症大鼠的微量白蛋白尿和肾脏组织病理学，并降低血清 TCH 浓度和 XOD 活性。四物汤和二妙散的组合也导致OAT1和OAT3表达比单独四物汤更大的增加。结论：四物汤和二妙散通过上调OAT1和OAT3协同改善大鼠高尿酸血症和肾损伤。
Background/Aims: Hyperuricemia is an independent risk factor for chronic kidney disease and cardiovascular disease. Here, we examined the combined protective effects of Chinese herbal formula Si-Wu-Tang and Er-Miao-San on hyperuricemia and renal impairment in rats. Methods: Rats were randomly divided into normal rats, hyperuricemic rats, and hyperuricemic rats orally administrated with benzbromarone (4.5 mg·kg-1·d-1), Si-Wu-Tang (3.78 g·kg-1·d-1) and Si-Wu-Tang plus Er-Miao-San (6.48 g·kg-1·d-1) for 4 weeks. Hyperuricemic rats were orally gavaged with adenine (0.1 g·kg-1·d-1) and potassium oxonate (1.5 g·kg-1·d-1) daily for 4 weeks. Serum uric acid, creatinine, total cholesterol (TCH), triglyceride and blood urea nitrogen (BUN) concentrations, as well as urinary uric acid and microalbuminuria were measured weekly. Serum xanthine oxidase (XOD) activity and renal histopathology were also evaluated. The renal expression of organic anion transporter 1 (OAT1) and organic anion transporter 3 (OAT3) was detected by western blot. Results: Si-Wu-Tang plus Er-Miao-San lowered serum uric acid, creatinine, triglyceride and BUN levels to a greater degree than did Si-Wu-Tang alone. Si-Wu-Tang plus Er-Miao-San ameliorated microalbuminuria and renal histopathology, as well as decreased serum TCH concentration and XOD activity in hyperuricemic rats. Combination of Si-Wu-Tang and Er-Miao-San also led to a greater increase in OAT1 and OAT3 expression than did Siwutang alone. Conclusion: Si-Wu-Tang and Er-Miao-San synergistically ameliorated hyperuricemia and renal impairment in rats through upregulation of OAT1 and OAT3.
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影响因子：
2.9
作者：
Bobulescu IA;Moe OW
通讯作者：
Moe OW
影响因子：
7.5
作者：
Liu M;Ravula R;Wang Z;Zuo Z;Chow MS;Thakkar A;Prabhu S;Andresen B;Huang Y
通讯作者：
Huang Y
影响因子：
13.2
作者：
Kramer, HM;Curhan, G
通讯作者：
Curhan, G
影响因子：
2.7
作者：
Haiyan Yan;Ying Ma;Mei Liu;Lan-lan Zhou
通讯作者：
Haiyan Yan;Ying Ma;Mei Liu;Lan-lan Zhou
影响因子：
1.7
作者：
J. Yamahara;H. Matsuda;Y. Naitoh;H. Fujimura;Y. Tamai
通讯作者：
J. Yamahara;H. Matsuda;Y. Naitoh;H. Fujimura;Y. Tamai