Bone-resorbing osteoclasts reveal a dynamic division of basal plasma membrane into two different domains.
Bone-resorbing osteoclasts reveal a dynamic division of basal plasma membrane into two different domains.
复制标题
骨吸收破骨细胞揭示了基底质膜动态划分为两个不同的域。
作者：
J. Salo;K. Metsikkö;H. Palokangas;P. Lehenkari;H. Väänänen
骨吸收的多核破骨细胞表现为一皱褶状的边缘膜贴骨和一接触循环的基膜。一种称为密封区的连接复合物将这两个膜域分开，但这些膜域的定义性质仍然不清楚。我们现在表明，使用包膜病毒糖蛋白和凝集素作为工具，破骨细胞在基底表面表现出一种新的膜结构域，当它们被极化吸收时。流感血凝素是上皮细胞的顶端靶点，它的靶点是基底表面顶部的一个有限区域，而水疱性口炎病毒g蛋白是上皮细胞的基底侧靶点，它占据了基底表面的其余部分。这些病毒糖蛋白既不聚集在褶边也不聚集在封闭区，但它们以一种特定的方式共享基面。为了证明基底膜分裂成两个不同的区域也发生在未感染的细胞中，我们分析了这些病毒受体的分布和一些凝集素的结合位点。这两种方法都表明，一些内源性蛋白位于活性破骨细胞基底表面的不同区域。我们还表明，这两种不同的膜结构域可以在扫描电镜水平上区分，因为中央基底结构域的绒毛外观。
Bone-resorbing multinucleate osteoclasts exhibit a ruffled border membrane apposing the bone and a basal membrane contacting the circulation. A junctional complex called the sealing zone separates these two membrane domains, but the defined nature of these membrane domains has remained obscure. We now show, using enveloped viral glycoproteins and lectins as tools, that osteoclasts exhibit a novel membrane domain in the basal surface when they are polarized for resorption. Influenza haemagglutinin, which is apically targeted in epithelial cells, is targeted to a restricted area at the top of the basal surface, while vesicular stomatitis virus G-protein which is basolaterally targeted in epithelia, occupies the rest of the basal surface. Neither of these viral glycoproteins is gathered to the ruffled border nor sealing zone area, but they share in a specific way the basal surface. To show that the division of basal membrane into two different domains also occurs in non-infected cells, we have analyzed the distribution of receptors for these viruses and binding sites of some lectins. Both of these methods show that also some endogenous proteins are located in different domains in the basal surface in active osteoclasts. We also show that these two different membrane domains can be distinguished in scanning electron microscopy level due to the villus appearance of the central basal domain.
影响因子：
56.9
作者：
RODRIGUEZBOULAN, E;NELSON, WJ
通讯作者：
NELSON, WJ
影响因子：
56.9
作者：
BLAIR, HC;TEITELBAUM, SL;GLUCK, S
通讯作者：
GLUCK, S