Polyoxazoline multivalently conjugated with indocyanine green for sensitive in vivo photoacoustic imaging of tumors.
Polyoxazoline multivalently conjugated with indocyanine green for sensitive in vivo photoacoustic imaging of tumors.
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DOI：
10.1038/srep33798
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发表时间：
2016-09-26
影响因子：
4.6
通讯作者：
Saji H
中科院分区：
文献类型：
--
作者：
Kanazaki K;Sano K;Makino A;Homma T;Ono M;Saji H
光声成像，使高分辨率成像在深部组织，最近引起了相当大的关注。对于肿瘤成像，已经提出了光声探针来增强光声效应以提高检测灵敏度。在这里，我们评估的可行性，使用生物相容性的亲水性聚合物，聚恶唑啉，共轭吲哚菁绿色（ICG）作为肿瘤靶向的光声探针通过增强的渗透性和保留效果。ICG分子多价共轭部分水解的聚恶唑啉，从而作为高灵敏度的光声探针。有趣的是，在相同的ICG浓度下，将多个ICG分子负载到聚恶唑啉上显著增强了光声信号强度。使用荷瘤小鼠的体内生物分布研究表明，与ICG缀合的5%水解的聚恶唑啉（50 kDa）（ICG/聚恶唑啉= 7.8），P14-ICG 7.8显示出相对高的肿瘤积累（9.4%ID/g），导致在测试的探针中递送最高剂量的ICG。P14-ICG7.8能够在给药后24小时通过光声成像清晰地可视化肿瘤区域;光声信号与注射剂量成比例地增加。此外，光声图像中血管中的信号强度没有显示出太大的变化，这归因于P14-ICG7.8的高肿瘤与血液比率。这些结果表明，聚恶唑啉-ICG将作为一个强大的探针灵敏的光声肿瘤成像。
Photoacoustic imaging, which enables high-resolution imaging in deep tissues, has lately attracted considerable attention. For tumor imaging, photoacoustic probes have been proposed to enhance the photoacoustic effect to improve detection sensitivity. Here, we evaluated the feasibility of using a biocompatible hydrophilic polymer, polyoxazoline, conjugated with indocyanine green (ICG) as a tumor-targeted photoacoustic probe via enhanced permeability and retention effect. ICG molecules were multivalently conjugated to partially hydrolyzed polyoxazoline, thereby serving as highly sensitive photoacoustic probes. Interestingly, loading multiple ICG molecules to polyoxazoline significantly enhanced photoacoustic signal intensity under the same ICG concentration. In vivo biodistribution studies using tumor bearing mice demonstrated that 5% hydrolyzed polyoxazoline (50 kDa) conjugated with ICG (ICG/polyoxazoline = 7.8), P14-ICG7.8, showed relatively high tumor accumulation (9.4%ID/g), resulting in delivery of the highest dose of ICG among the probes tested. P14-ICG7.8 enabled clear visualization of the tumor regions by photoacoustic imaging 24 h after administration; the photoacoustic signal increased in proportion with the injected dose. In addition, the signal intensity in blood vessels in the photoacoustic images did not show much change, which was attributed to the high tumor-to-blood ratios of P14-ICG7.8. These results suggest that polyoxazoline-ICG would serve as a robust probe for sensitive photoacoustic tumor imaging.
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用于活体小鼠高灵敏度和多重研究的增强型光声成像剂系列。
影响因子：
17.1
作者：
de la Zerda A;Bodapati S;Teed R;May SY;Tabakman SM;Liu Z;Khuri-Yakub BT;Chen X;Dai H;Gambhir SS
通讯作者：
Gambhir SS
影响因子：
4.6
作者：
Luxenhofer, Robert;Han, Yingchao;Schulz, Anita;Tong, Jing;He, Zhijian;Kabanov, Alexander V.;Jordan, Rainer
通讯作者：
Jordan, Rainer
影响因子：
10.8
作者：
Gaertner, Florian C.;Luxenhofer, Robert;Essler, Markus
通讯作者：
Essler, Markus
影响因子：
3.6
作者：
Jo, Jun-ichiro;Nagane, Kentaro;Tabata, Yasuhiko
通讯作者：
Tabata, Yasuhiko
荧光猝灭纳米探针致力于体内光声成像和深部组织高效肿瘤治疗
影响因子：
13.3
作者：
Qin, Huan;Zhou, Ting;Xing, Da
通讯作者：
Xing, Da