CD44 variant isoforms in head and neck squamous cell carcinoma progression.
CD44 variant isoforms in head and neck squamous cell carcinoma progression.
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DOI：
10.1002/lary.20506
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发表时间：
2009-08
期刊：
影响因子：
2.6
通讯作者：
Bourguignon, Lilly Y. W.
中科院分区：
文献类型：
--
作者：
Wang, Steven J.;Wong, Gabriel;de Heer, Anne-Martine;Xia, Weiliang;Bourguignon, Lilly Y. W.
CD 44受体家族包括多种变体亚型，其中一些已与恶性特性（包括迁移、侵袭和转移）有关。本研究旨在探讨CD 44 v3、v6和v10变异亚型在头颈部鳞状细胞癌（HNSCC）肿瘤进展行为中的作用。涉及细胞培养和临床组织标本的实验室研究。在HNSCC细胞系HSC-3中分析标准CD 44 ε和CD 44变体同种型v3、v6和v10的表达。确定了CD 44亚型在迁移、增殖和顺铂耐药中的作用。对82例HNSCC患者的临床组织标本进行免疫组化分析。分析原发性肿瘤标本（n=82）和转移性颈淋巴结（n=24）中标准CD 44 s和CD 44 v3、v6和v10变体的表达与各种临床病理变量的关系。HSC-3细胞表达至少4种CD 44同种型，并且这些CD 44同种型介导迁移、增殖和顺铂敏感性。与原发性肿瘤相比，转移性淋巴结中CD 44 v3强表达的比例显着更大（淋巴结：14/24 vs.原发性肿瘤：38/82），CD 44 v6（淋巴结：18/24 vs.原发性肿瘤：26/82）和CD 44 v10（淋巴结：14/24 vs.原发性肿瘤：16/82），而标准CD 44的表达在转移性淋巴结和原发性肿瘤中没有显著差异（淋巴结：10/24 vs.原发性肿瘤：60/82）。CD 44变异体亚型的表达与晚期T分期（v3和v6）、区域（v3）和远处（v10）转移、神经周围浸润（v6）和放射治疗失败（v10）相关。CD 44 v6和CD 44 v10也与较短的无病生存期显著相关。CD 44亚型介导HNSCC的迁移、增殖和顺铂敏感性。此外，某些CD 44变体的表达可能是HNSCC进展的重要分子标志物，并且应该作为潜在的治疗靶点进行研究。
The CD44 family of receptors includes multiple variant isoforms, several of which have been linked to malignant properties including migration, invasion, and metastasis. The objective of this study was to investigate the role of the CD44 v3, v6, and v10 variant isoforms in head and neck squamous cell carcinoma (HNSCC) tumor progression behaviors. Laboratory study involving cell cultures and clinical tissue specimens. Analysis of the expression of standard CD44s and the CD44 variant isoforms v3, v6, and v10 was carried out in the HNSCC cell line, HSC-3. The role of CD44 isoforms in migration, proliferation, and cisplatin resistance was determined. Immunohistochemical analysis was performed on clinical tissue specimens obtained from a series of 82 HNSCC patients. The expression of standard CD44s and the CD44 v3, v6, and v10 variants in primary tumor specimens (n=82) and metastatic cervical lymph nodes (n=24) were analyzed with respect to various clinicopathologic variables. HSC-3 cells express at least 4 CD44 isoforms, and these CD44 isoforms mediate migration, proliferation, and cisplatin sensitivity. Compared to primary tumors, a significantly greater proportion of metastatic lymph nodes demonstrated strong expression of CD44 v3 (lymph node: 14/24 vs. primary tumor: 38/82), CD44 v6 (lymph node: 18/24 vs. primary tumor: 26/82), and CD44 v10 (lymph node: 14/24 vs. primary tumor: 16/82), while expression of standard CD44 was not significantly different in metastatic lymph nodes and primary tumors (lymph node: 10/24 vs. primary tumor: 60/82). Expression of CD44 variant isoforms were associated with advanced T stage (v3 and v6), regional (v3) and distant (v10) metastasis, perineural invasion (v6), and radiation failure (v10). CD44 v6 and CD44 v10 were also significantly associated with shorter disease-free survival. CD44 isoforms mediate migration, proliferation, and cisplatin sensitivity in HNSCC. Furthermore, expression of certain CD44 variants may be important molecular markers for HNSCC progression, and should be investigated as potential therapeutic targets for therapy.
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影响因子：
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作者：
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通讯作者：
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影响因子：
4.8
作者：
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DOI：
10.1001/archotol.126.10.1217
发表时间：
2000-10-01
影响因子：
--
作者：
Kanke, M;Fujii, M;Matsumura, Y
通讯作者：
Matsumura, Y